The Expression And Significance Of Fascin And VEGF In Transitional Cell Carcinoma Of The Bladder

Background and Objects:The bladder tumor is the one of most common malignant tumor.the biological feature is multicentre, easy relaPse, and infiltrative growth.the invasion and metastasis of tumor is the leading cause of death in Patient. Now,the mechanism of the growth and Progression of BTCC is still ambiguity and there is no sPecific marker that is used to diagnose BTCC and judge the Prognosis of BTCC.Fascin, a structuarlly unique and evolutionarily conserved actin-bunding Protein that is found in membrane ruffles, microsPikes, and stress fiber, induces membrane Protrusions and increases cell motility in various transformed cells.We found Fascin usually had uP-regulation in tumors from epithelial tissue. Fascin protein, recently, is often reported to be found in tumors,such as esophageal squamous cell carcinoma, gastric carcinoma, colon carcinoma, ovarian cancer and so on.However,there is no report over Fascin Protein in bladder cancer in china,little in foreign country. By studying the immunoreactivity of Fascin and VEGF and analysising clinicopathlogical index in BTCC, my experiment investigate the effect of Fascin protein on biological behaviour and correlation with VEGF in order to judge the possibility Fascin being a useful marker for the malignant BTCC.Materials and Method:Ten samples from normal bladder tissue and 56 samples from transitional cell carcinoma of bladder were collected. Bladder cancer sampleswere obtained from surgically-resected sPecimens from the Second Affiliated HosPital of Tianjin University between 2004 and 2008.The Patients had no Prior chemotheraPy or radiotheraPy.All these cancers were diagnosed pathologically. The expression of Fascin and VEGF was examined by SABC (StrePtAvidin-Biotin Complex) immunohistochemistry in 56 Paraffin-embedded tissue specimens and 10 control groups of normal bladder tissues with analysis by SPSS 13.0Results:l.In cancer cells, Fascin and VEGF expression were found mainly in the cytoplasm.The positive expression rate of Fascin and VEGF in normal bladder tissue was 0%, while the positive expression rate of Fascin and VEGF was statistically significant(P<0.01).2. The Positive expression of Fascin in transitional cell carcinoma of bladder had no distinct difference in age, sex, tumorsize and tumor quantity(P>0.05), but it had a close correlation with the degrees of histological grades(P<0.01), clinical stages (P<0.01), and Primary or recurrent carcinoma(P<0.01).3.The Positive expression of VEGF in transitional cell carcinoma of bladder had no distinct difference in age, sex, tumorsize and tumor quantity(P>0.05), but it had a close correlation with the degrees of histological grades (P<0.01), clinical stages (P<0.05), and Primary or recurrent carcinoma (P<0.01). 4. The Positive expression of Fascin and VEGF in 56 cases of transitional cell carcinoma of bladder was closely associated with each other. The expression of Fascin was closely correlated with that of VEGF (liner index of Pearson=0.4769, P<0.01)Conclusion:1.The expression of Fascin increases in high-grade bladder transitional cell carcinoma and the over-expression of Fascin is correlated with the stage of invasive tumor.It Provides referencing index and the development and invasion of bladder carcinoma.2.The expression of VEGF increases in high-grade bladder transitional cell carcinoma and the over-expression of VEGF is correlated with the stage of invasive tumor.3.A close correlation between expression of VEGF and Fascinin transitional cell carcinoma of bladder was observed. It is indicated that there is certain regulation bewteen Fascin and VEGF. They contribute to the angiogenesis and metastasis of bladder carcinoma in coordination.4.It is suggested that the Positive experssion of Fascin and VEGF might be the referencing indxe of the development and invasion of bladder carcinoma. It is shown thatFascin and VEGF may provide theoretical fundation of chemoPreventive stategy for bladder cancer in the future.