The Relationship Of Genetic Polymorphisms Of Lymphotoxin-a And Myeloperoxidase With Acute Coronary Syndrome

Objectives:To study the distribution and characteristics of lymphotoxin-a gene A252G polymorphism and myeloperoxidase levels in patients with acute coronary syndrome, and then explore the relationship between genetic factors, inflammatory response and the morbility of acute coronary syndrome.Methods:We had slected 180 subjects who hospitalized in cardiovascular department of Tianjin first central hospital from Oct 2007 to Oct 2008. Their clinic informations were recorded, such as gender, age, smoking, drinking, CHD family history, diabetes history, triglyceride, total cholesterol, low-density lipoprotein cholesterol, high density lipoprotein cholesterol, uric acid, fasting plasma glucose, etc. And, all subjects were undergone with coronary angiography. Unstable angina pectoris(UAP) and acute myocardial infarction(AMI) were both diagnosised basing on ACC/AHA Guidelines. According to the results of coronary angiography,180 subjects were divided into ACS group (100 cases) and control group (80 cases). And ACS group was divided into UAP and AMI subgroup. Their serum levels of Myeloperoxidase were measured with enzyme-linked immunosorbent assay (ELISA). DNA was extracted with AxyPrep DNA reagent. The mutation of the LTA 252A→G transition was examined by polymerase chain reaction and identified with agarose gel electrophoresis. Using statistic software SPSS 13.0 to analyze the datas, Chi square test were used to compare the two groups'numeration datas. Measurement data were expressed with mean±standard deviation(x±s), t tests were used to compare the numbers of two samples,and analysis of variance were used to compare the numbers of multi-samples. P<0.05 was considered to be statistic significance.Results:1. The subjects'clinical characteristics:It can be seen that CHD family history, triglyceride, low-density lipoprotein cholesterol, high density lipoprotein cholesterol, uric acid, plasma glucose(P<0.01), smoking and hypertension history (P<0.05) were significantly different between the ACS group and the control group. However, gender, age, drinking, total cholesterol were not significantly different betweem the ACS and control patients(P>0.05). 2. The level of MPO in ACS group was significantly higher than control group (P<0.05). There were no differences of MPO between AMI group and UAP group(P>0.05). There were no differences of MPO in ACS group with the different coronary lesion(P>0.05). There were three kinds of genotypes AA, AG and GG in LTA gene 252. LTA gene A252G genotype frequencies and distributions were different between ACS group and control group. Genotype of AG was highest in ACS group. There were no differences of LTA gene A252G genotype frequencies and distributions in patients with different coronary lesions of ACS group. There were no differences of MPO with different genotype of LTA gene A252G in ACS and control group.Conclusions:1,The levels of MPO in ACS group were higher than that in control group, it may be a marker for plaque rupture.2, The mutation of LTA gene A252G was associated with ACS. May be it is one of risk factors of coronary heart disease.