Association Between The Myeloperoxidase Gene-463G/A Polymorphism And Coronary Heart Disease

Objective To explore the relationship between the myeloperoxidase (MPO) —463G/A gene polymorphism and coronary heart disease(CHD) in Lanzhou district of Han people.Methods 171 patients with CHD and 111 controls were genotyped for the MPO—463G/A polymorphism by polymerase chain reaction restriction fragment length polymorphism (PCR- RFLP) analysis in a case-control study. The association between MPO genotype and CHD were analyzed.Results1. The MPO —463 G/A genotype frequencies among the controls were G/G 0.640, G/A 0.342 and A/A 0.018, respectively, the allele frequencies of G and A were 0.811 and 0.189. Hardy -Weinberg equilibrium was tested with expected frequencies in cases and controls.₂. Assumed the effects of the A allele to be dominant, the G/A+A/A genotype frequencies in CHD group, as well as UAP/NSTEMI subgroup, were lower than controls (p<0.01). A allele frequency was also lower in CHD group (p<0.05) and UAP/NSTEMI subgroup (p<0.01) than controls.3. Study on relationship between MPO genotypes and the number of diseased vessels discovered that the distribution of G/G and G/A were difference among simplex ramus, double and multitude rami diseased vessels groups(p<0.01), as well as the allele frequencies of G and A (p<0.05).4. Logistic regression analysis showed that the gender, age, smoking status and diabetes mellitus were associated with higher risk of CHD and high-density lipoproteins, MPO—463G/A mutation was protective factors.ConclusionThe results suggest that the MPO—463G/A polymorphism associated with the CHD. MPO—463G/A mutation was protective factors. A allele is maybe associated with a lower risk of CHD.