| Objective:To study the effect of resveratrol on the cerebral infarction and behavior in rats after focal cerebral ischemia-reperfusion injury. Based on this model, we also explore the possible mechanisms of the effect. Moreover, to investigate the effect of resveratrol on the viability and morphology of cell by establishing the oxygen-glucose deprivation (OGD) model in PC 12 cells.Methods:(1)Rat model of focal cerebral ischemia reperfusion injury made by occlusion of middle cerebral artery was used. In the dose-dependence study, rats were randomly divided into 7 groups:model group, sham-operated group, positive control group, and resveratrol-treated groups (5mg/kg, 10mg/kg,20mg/kg and 40mg/kg). The size of cerebral infarction, the extent of edema and the water content were measured in the rats. (2)In the long-term experiment, the rats were randomly divided into 6 groups:model group, sham-operated group, positive control group, and resveratrol-treated groups of high, medium and low doses. Postural reflex test and limb use asymmetry test were performed as behavioral indexes on 2th,4th,6th and 8th day after the rats were subjected to focal cerebral ischemia-reperfusion injury for evaluating the protective effect of resveratrol on the brain damage. (3) By establishing oxygen-glucose deprivation (OGD) model of PC 12 cells in vitro, we observed the change of the cells. At the same time, MTT assay was applied to evaluate the cell's viability and biochemical method was used to determine LDH activity.the contents of nitric oxide(NO) and the malondialdehyde(MDA). the activity of superoxide dismutase(SOD).Results:(1) Compared with model group(28.30±7.92), cerebral infarction area decreased obviously:19.21±14.72,15.32±13.53,14.47±10.85,13.74±12.89. Compared with model group(65.81±1.75),the extent of brain edema decreased obviously:64.27±1.45,63.91±1.53,63.68±1.80,63.57±1.65.The 5mg/kg resveratrol-treated group had no significant effect.(2) Behavior evaluation results from the relative long-term treatment study showed that resveratrol treatment remarkably improved the limb movement of rats with cerebral ischemia-reperfusion injury with statistical significance.i the weight variation ratio:The weight of rats in the sham-operated group were significantly increased,except On dl, The weight of rats in the model group were significantly decreased.compared with sham-operated group in same time, On 2th,4th,6th and 8th day the weight variation ratio after focal cerebral ischemia and reperfusion injury in the model group were significantly increased (-7.61±5.71,-10.57±10.49,12.40±12.29,12.05±13.52), compared with the model group,On 2th,4th and 6th day of the weight test, the resveratrol middle dose (10mg/kg) group were significantly decreased(-9.07±3.85,-9.44±11.97,-10.90±15.82,), On 4th and 6th day of the weight test, the resveratrol high dose (20mg/kg) group were significantly decreased(-12.15±12.80,-13.25±13.89,),the Positive control group were significantly decreased (-9.58±3.09,-9.12±6.56,-9.43±7.67,-7.50±8.46). ii Postural reflex test:On the Postural reflex test neurological score,compared with sham-operated group(0±0), On 2th,4th,6th and 8th day the model group were significantly increased(1.42±0.48,1.36±0.33, 1.29±0.51,0.91±0.30). compared with the model group,the resveratrol high dose (20mg/kg) group were significantly decreased(1.01±0.37,0.78±0.42,0.77±0.28, 0.59±0.39,). On 4th day the resveratrol low dose (5mg/kg) and middle dose(10mg/kg) group were significantly decreased(1.06±0.29,1.01±0.30),the Positive control group were significantly decreased (1.08±0.31,0.78±0.31,0.87±0.63,0.64±0.44).iii limb use asymmetry test:On limb use asymmetry test score, compared with sham-operated group(9.5±21.51,-0.75±25.77,4.25±23.64,4±24.42),On 2th,4th,6th and 8th day the model group were significantly increased (66.43±32.78,69.29±23.69, 50.36±17.81,51.79±40.03),compared with the model group, On 2th,4th and 6th day the resveratrol high dose (20mg/kg) group were significantly decreased(35.67±34.69, 29±27.33,28.33±27.82), On 4th day the resveratrol low dose (5mg/kg) and middle dose (10mg/kg) group were significantly decreased(47.5±18.60,46.88±19.07), On 2th,4th and 6th day the Positive control group were significantly decreased (26.56±29.98,44.38±28.57,33.13±27.5). (3) In the cell test, the cellular morphology of PC12 cells were altered after OGD. resveratrol (12.5μM,25μM,50μM) could significantly decrease the injury of PC 12 by OGD. compared with the model group (70.45±2.94), the MTT value of resveratrol group increased obviously:74.2±3.88, 92.55±5.08,90.3±4.55. In PC12 cells, compared with the model group (705.85±30.65), LDH leakage of resveratrol group decreased obviously: 630.76±31.28,619.72±23.91,599.34±37.54. In PC12 cells, compared with the model group (11.09±1.1), MDA leakage of resveratrol group decreased obviously: 9.43±0.53,9.27±0.84,7.82±0.63. In PC12 cells, compared with the model group (16.69±0.3), SOD leakage of resveratrol group increased obviously:17.67±0.23,18.79±0.26,18.68±0.15. In PC12 cells, compared with the model group (42.25±0.92), NO leakage of resveratrol group decreased obviously:31.25±2.11,25.88±2.06,25.38±2.73.Conclusion:Resveratrol has protective effects on focal cerebral ischemia-reperfusion injury,which was demonstrated by significantly reducing the infarct size and brain edema and improves physical disabilities caused by ischemia. And resveratrol could exert a protective action on oxygen-glucose deprivation in PC12 cells.
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