The Study Of RRM2 In Endometrial Carcinoma In Vivo

Back ground and objective:The pre-study of the experiment has found that small interfere RNA of ribonucleotide reductase subunit M2 (RRM2) can inhibit the proliferation of Ishikawa cells and induce the apoptosis of the Ishikawa cells in vitro.This experiment is designed to study the inhibition of siRNA-RRM2 to the xenograft in vivo.Methods:Using the Ishikawa cells to form xenografts in the nude back, then the mice were separated into three teams:RRM2-siRNA group,negative control group, liposome group.Injecting pRNAT-CMV3.2-Neo-RRM2 wrapped by LipofectamineTM 2000, pRNAT-CMV3.2-Neo-neg wrapped by LipofectamineTM 2000 and equal volume of blank liposomes respectively into the xenografts for seven days, once a day.The volumes of xenografts were measured before and after the treatment. The inhibition ratio of tumor proliferation was calculated. The RRM2-mRNA expression was measured by RT-PCR and the RRM2-protein expression was measured by western blot. The data was analyzed by SPSS 13.0.Results:The mean tumors'weight of RRM2-siRNA group was (0.2600±0.0529)g, and the negative control group was (0.3867±0.0831)g,and the liposome group was (0.4017±0.0624)g.The mean tumors'volumes of the three groups before treatment were respectively (0.1011±0.0316) cm3, (0.0949±0.0254) cm3, (0.0954±0.0437) cm3.When the experiment was finished, they were respectively (0.1666±0.0481) cm3, (0.2375±0.0499) cm3, (0.2811±0.0168) cm3.The tumors'volume of the negative control group and liposome group were significantly increased than the RRM2-siRNA group. The difference was significant.While the difference between the negative control group and the liposome group was not significant(P=0.075). The tumors' expressions of RRM2mRNA and protein in RRM2-siRNA group were reduced than the other two groups.Conclusion:The siRNA-RRM2 can inhibit the growth of the xenograft in vivo.RRM2 could be used in the treatment of endometrial carcinoma.Objective:To investigate the inhibitory effect of cinobufotalin on the xenograft of nude mice of endometrial carcinoma cell line Ishikawa and the influence of ribonucleotide reductase subunit M2(RRM2). Methods:The nude mice with xenografts of endometrial carcinoma were divided randomly into two group.They were injected cinobufotalin with the dose 3g/Kg/d and stroke-physiological saline solution for 1 week respectively. The sizes of xenografts were measured before and after the treatment. The inhibition ratio of tumor proliferation was calculated. The RRM2-mRNA expression was measured by RT-PCR and the RRM2-protein expression was measured by western blot. The data was analyzed by SPSS13.0.Results:After 1 week, the size of xenografts of cinobufotalin group was (0.131 4±0.0304) cm3.It was lower than the control group which was (0.3600±0.1145) cm3(P <0.05).The inhibition ratio of tumor proliferation was38.87%.The difference of RRM2-mRNA levels between the cinobufotalin group and the control group was significant(P=0.019).The difference of RRM2-protein levels of the two groups was the same as the RRM2-mRNA levels (P=0.001)Conclusion:Cinobufotalin significantly inhibits the growth of the xenografts of endometrial carcinoma in nude mice by inhibiting the expression of RRM2.