The Research Of Transplantation In Myocardial Infarction Of Homogeneous Bone Marrow Mesenchymal Cells To Raise Peripheral Blood Stem Cell

Abstract:Background:Recent years, stem cells transplantation was one of the focus in cardiology. The study found that transplanted stem cells can survive,differentiation and improve heart function in damaged tissue, Possible mechanism was transplanted cells promote key enzymes or the nest and endogenous stem cell differentiation through the transplanted cells secrete or paracrine effect. Stem cells improved Na+-K+-ATP enzymes and Ca2+-ATP enzymes which we had researched, but it did not know if they can promote the nest. Objective:To observe whether they could improve heart function and recruit peripheral blood stem cells after local injected homogeneous bone marrow mesenchymal stem cells to myocardial infarction rats model and then intravenous peripheral blood stem cell. Methods:Thirty-two SD rats (180-200g) were randomly divided into 4 groups (8 rats each group), ie. normal group:did not made model; fake transplanted group:made myocardial infarction operation but injected DMEM; MSCs transplanted group: partly injected MSCs on myocardial infarction rats model; MSCs and EPCs transplanted group:partly injected MSCs and peripheral blood stem cell on myocardial infarction rats model. Respectively detected heart function at pre-and post-transplanted 5 weeks; Immunohistochemistry method to detect marked stem cells and whether bone marrow mesenchymal cells could raise peripheral endothelial progenitor cells. Analyzed these index through SPSS 13.0 statistical software. Result:(1) Comparison between pre-and post-transplantation of stem cell, normal group did not changed;fake transplanted groups all decreased in LVSP,LV±dp/dt max and increased in LVDP after 5 weeks (p<0.01),this indicated the worsen heart function; MSCs transplanted group and MSCs +EPCs transplanted group had improved in LVSP,LV±dp/dt max (p<0.01),but did not obviously in LVDP, this indicated the recovery heart function after 5weeks;(2) After 5weeks of stem cells transplanted, compared to normal group, a significant decreased in LVSP,LV±dp/dt max (p<0.01) and increased in LVDP (p<0.01) in fake transplanted group, this indicated myocardial got worse; MSCs transplanted group and MSCs+EPCs transplanted group also had decreased in LVSP,LV±dp/dtmax and increased in LVDP(p<0.01),this indicated the recovery of myocardial,but did not reach normal; compared to fake transplanted group, MSCs group and MSCs+EPCs transplanted group had significant increased in LVSP,LV±dp/dtmax (p<0.01) and decreased in LVDP (p<0.01), this indicated stem cells transplanted group better than fake group; compared between stem cells transplanted group, there did not obviously change in LVSP,LV±dp/dt max and LVDP (p>0.01),this indicated the small difference of them. (3)5 weeks later we detected BrdU marker at infarction areas, but did not detect DAPI marker,this indicated they do not gather. Conclusion:①Successfully established the model of myocardial infarction②There had obviously improved at 5weeks later③Did not obviously improved in MSCs+ EPCs transplanted group④Bone marrow mesenchymal cells did not raise endothelial progenitor cells.