The Relation Between Trational Chinese Medicine Type Of Syndrome In Nsopharyngeal Darcinoma And T-lymphocyte Subsets

Abstract:Objective:Explored the relevance among Nasopharyngeal carcinoma patients'four TCM type of syndromes (Phlegmy turbidity and gather pattern, Blood and gas condensation pattern, Fluid loss pattern, Spleen and stomach qi deficiency pattern) and T-lymphocyte (CD3, CD4, CD8, CD4/CD8), to search for an objective basis to differentiation the nasopharyngeal carcinoma patients'TCM type of syndrome and the reference for comprehensive treatment of nasopharyngeal carcinoma.Methods:Random Sample 20 cases of NPC patients that was admitted without any treatment, and must have clinical and pathological diagnosis by the Luzhou Medical College Affiliated hospitals.The samples are also with TCM type of syndrome that we are need, which Phlegmy turbidity and gather and Blood and gas condensation pattern,20 cases of the condensation, aged 18-70 years; Spleen and stomach qi deficiency pattern that appeared after chemotherapy,20 cases; Fluid loss pattern that appeared after radiotherapy,20 cases. Control group:20 healthy volunteers, aged 18-70 years.Every case must fasting 12 hours, collect venous blood 2ml with EDTA anticoagulation in the next morning,Fluorescent marker directly and Flow cytometric detection peripheral blood mononuclear cells (PBMC) membrane surface CD3, CD4, CD8. Put CD3/CD4/CD8 three-color fluorescent monoclonal antibody that produced by the United States BECKMAN COULTER company 20μl in 100μl peripheral blood that with EDTA anticoagulation, incubated 20 min at room temperature, then put 650μl red blood cell lysate in the sample,wait 3min and joined the cell protective solution 265μl,at last detect the sample by machine. Pre-test calibration FLI, FL2, FL4, and SS, FS channel'accuracy by standard fluorescent microspheres Flow-check, make sure each channel CV<2%. Finally analysis T-lymphocyte (CD3, CD4, CD8, CD4/CD8) expression levels of each TCM type of syndrome by Statistics and the relationship between the control group.Results: The two TCM type of syndrome patients'before treatment CD4 expression levels and CD4/CD8 are decrease compared with healthy, CD8 expression level increase slightly compared with healthy people, but in the two TCM type of syndrome patients'before treatment, blood and gas condensation pattern patients'CD3 expression levels is slightly lower than the healthy, and phlegmy turbidity and gather pattern patients'CD3 expression levels is slightly increase than the healthy; Spleen and stomach qi deficiency patients'that appear after chemotherapy'CD3, CD4 expression levels compared with healthy rise highly, CD8 expression level compared with healthy people is significantly lower, all the results have no significant difference (P>0.05), Spleen and stomach qi deficiency patients'compared with healthy people, their CD4/CD8 also is significantly increased,And it has a statistically significant difference (P<0.05); the Fluid loss pattern patients'occurring after radiation therapy compared with the healthy,the CD3 expression level is lower, CD8 expression level is increase, but no significant difference (P> 0.05), but the expression level of CD4 and CD4/CD8 are significantly reduced compared with healthy people, there are significant difference (P<0.05);in the TCM type of syndrome patients'that before treatment and after treatment,the Fluid loss pattern patients'CD3 expression level is the lowest, phlegmy turbidity and gather pattern patients' and Spleen and stomach qi deficiency patients'CD3 expression levels are similar,and higher than blood and gas condensation pattern patients'and Fluid loss pattern patients', blood and gas condensation pattern patients'CD3 expression level between Fluid loss pattern patients'and phlegmy turbidity and gather pattern patients'(or Spleen and stomach qi deficiency patients'),but the results no statistically significant (P> 0.05),in the two TCM type of syndrome patients'before treatment, phlegmy turbidity and gather pattern patients'CD4 expression level higher than blood and gas condensation pattern patients',both CD8 expression levels and CD4/CD8 are similar,all the results have no statistically significant (P> 0.05),the Spleen and stomach qi deficiency patients' CD4 expression level and CD4/CD8 are significantly higher than both TCM type of syndrome patients'before treatment and the Fluid loss pattern patients' after radiationtherapy,but the CD8 expression level is significantly lower than both TCM type of syndrome patients'before treatment and the Fluid loss pattern patients'after radiationtherapy,all the results have a significant difference (P<0.05),the CD4 expression level and CD4/CD8 of the Fluid loss pattern patients'after radiationtherapy are significantly lower than both TCM type of syndrome patients'before treatment and Spleen and stomach qi deficiency patients'after Chemotherapy,but the CD8 expression level is significantly higher than both TCM type of syndrome patients'before treatment and Spleen and stomach qi deficiency patients'after Chemotherapy, all the results have a significant difference (P<0.05).Conclusion:1. The CD3 expression level change little at Healthy people and all Nasopharyngeal carcinoma patients'TCM types of syndromes, so CD3 expression level has less relative to the TCM type of syndrome.2. Nasopharyngeal carcinoma patients'CD4, CD8 expression level and CD4/CD8 change obvious at before and after treatment appear the TCM types of syndrome, can be used as discrimination the TCM type of syndromes'objective basis.3. The expression of CD4,CD8 and CD4/CD8 change little in the Healthy group as compared with two TCM types of syndrome group that appear treatment; the expression of CD4 and CD8 change little in the Healthy group as compared with Spleen and stomach qi deficiency pattern group that appear after chemotherapy,but the CD4/CD8 change obvious; the expression of CD4,CD8 and CD4/CD8 change obvious in Healthy as compared with Fluid loss pattern group.therefore,the T-lymphocyte can't as a independent basis to distinguish healthy people between NPC'patients or all kinds NPC'TCM types of syndrome.4. T-lymphocyte in nasopharyngeal carcinoma TCM type of syndromes differentiation has great value, the discriminant function established by the review-type test, the judge to toverall rate of 58.0%, indicating the discrimination to nasopharyngeal carcinoma TCM type of syndromes relatively good, with some reference value, for the TCM clinical diagnose provides a good objective basis.