Lactobacillus Cervical Cancer Hela Cell Inhibition Mechanism Of Lactobacilli Against Cervical Cancer Hela Cell Inhibition Mechanism

Objective: Lactic acid bacteria observed in cervical cancer cell lines Hela cell line, Lactobacillus further explore the role of cervical cancer cell killing mechanisms So as to develop safe, effective, low-cost prevention and treatment of cervical cancer drug basis.Methods:1 Placental blue staining were observed in the oil and Lactobacillus counts in different states of Hela cells and cervical cancer cell lines were cultured at different time stages of the adhesion index and changes in cell viability after adhesion.2 Using flow cytometry (antibody direct immunofluorescence) cervical cancer cells by Lactobacillus on the surface of the table type (CD80, CD86, MHC-1) effects.3 MTT was observed with Lactobacillus immune cells (NK cells, CTL cells) in cervical cancer cell killing effects.Results:1 Lactic acid bacteria and Hela cervical cancer cell lines and cell viability after the adhesion index results:1.1 Oil microscope Lactobacillus inhibited the adhesion of Hela cells: Tumor cells cultured for 12 hours, medium are clear, and control cell growth-intensive, growth in good condition, good gloss; Cell extension, irregular spindle. The density of Lactobacillus cells compared with control group, decreased gloss. Inactivated Lactobacillus group on the adhesion of Hela cell density was significantly higher than viable group, cell viability better. After 48 hours, see the control cells, have overlapping growth of Lactobacillus cells, cells appeared less sparse, destruction of cell morphology, cell shape, cell shrinkage, smaller size, cell gap increases, and round. Membrane rupture, swelling. Inactivated group more significant than the viable group, and the more obvious changes with time. Adhesion index of .1.2 compared two groups of lactobacilli, Lactobacillus viable group adherence index increased gradually with culture time, A peak at 12 hours of incubation, and then with time, adhesion decreased gradually Inactivated and viable group was similar, in every culture time compared with inactivated Lactobacillus adhesion index group were higher than viable group.1.3 Lactobacillus adhesion Hela cell viability after the comparison: in the co-culture 1 h, 3 h, 6 h phase difference between the two groups no Hela cell viability, 12h in culture, the vitality of a sudden drop in viable cells, while the activity of cells inactivated remained at a high level. Then with time, inactivation of group co-cultured cancer cell viability began to decrease 24h and less than the viable group (P <0.01).2 actic acid bacteria on the cancer cell surface antigen (MHC-1 molecules, CD80 and CD86 molecules) expression assay (flow cytometry): Cells in the negative control group well grew adhesively,most in shuttle shape,moderately sized,clear nuceolus,nulear division phase found.Cells shrinkage after treatment by acitretin,the breakdown was irregular in shape,some cells appear at both ends of the pseudo-foot slender.INF-stimulated cells showed the number of bipolar cells increase.The cells treated by acitretin and INF were present in the supernatant.The density of adherence cells was diminished when compared with the control.2.1 Lactobacillus different states of cervical cancer cell surface antigen expression: The role of lactic acid bacteria Lactobacillus two states after the group's cervical cancer cell surface antigen expression were significantly higher. Inactivated bacteria group and viable group to promote the role of cervical cancer cell surface antigen expression in a significant difference, and after inactivation of the role of Lactobacillus Hela cervical cancer cell surface antigen expression was significantly higher than the viable group.2.2 in control group and the Lactobacillus cervical cancer cell surface antigen expression: Control cervical cancer cell surface antigen (MHC-1, CD80, CD86) expression was extremely low. Control cervical cancer cell surface antigen (MHC-1, CD80, CD86) expression was extremely low. The role of lactic acid bacteria Lactobacillus two states after the group's cervical cancer cell surface antigen expression were significantly higher (P <0.05).3 Determination of lactic acid bacteria 3.MTT regulate immune cells in cervical cancer cell killing effect of:3.1 The role of different concentration of lactic acid bacteria after the NK cells, CTL cell killing activity of Hela cells results show that: Lactobacillus markedly enhanced the role of NK cells after, CTL cell killing activity of cervical cancer Hela cells, and NK cells, CTL cells with different concentrations of the role of lactic acid bacteria killing activity after the significant difference (P <0.05). To effectively target ratio is 20:1 strongest cytotoxic activity, with concentration-dependent.3.2 The role of different time stages after Lactobacillus NK cells, CTL cell killing activity of Hela cells results show that: NK cells, CTL cells with Lactobacillus different time of their killing activity of significant differences, and with time (12,24,48,72) the killing activity increases, and there are time-dependent.Conclusion:1 Lactobacillus well adhesion and inhibit cervical Hela cell growth, live Lactobacillus cervical cells and cervical cell has better adhesion inhibition and has time dependent.2 Lactobacillus could upregulate cervical cell surface antigen CD80, CD86, MHC-1 expression inactivated Lactobacillus role cervical carcinoma after Hela cell surface antigen expression rate significantly higher than viable group.3 Using MTT method results show Lactobacillus can promote immune cells (NK cell, CTL cells) on cervical Cells and dose - time dependent.