The Study Of Correlation Between The Polymorphisms Of Apoai , MnSOD And Blood Biochemical Indicators In Community Resi Dents Of Shijiazhuang

Objective: The prevalence rate of Alzheimer's disease (AD) increased year by year as population aging. AD had become a common neurodegenerative disorders of elderly people. In the clinical situation , this disease was in the late phage once it was diagnosed, so far there were no specific drugs and treatments. Therefore, early diagnosis and early intervention have became a research hotspot in AD. And AD-related gene polymorphism in pathogenesis had been the focus on the study area. One of hot spots was Apolipoprotein AI (APOAI) and manganese superoxide dismuAtaPsOe (AMI nwSaOs Da )m gaejnoer . c o mponent of high density lipoprotein. Moreover, it was an important in vivo apolipoprotein. It could promote the selective uptake of lipids and cholesterol outflow, and participate in reverse cholesterol transport. It was a very important role in the human body for cholesterol metabolism. It was mainly synthesized in the liver and small intestine and also expressed in vascular endothelium of brain. There was reported that A allele carriers of APOAI-75bp polymorphism increased the risk of early onset Alzheimer's disease (EOAD) significantly. Study shown that hypercholesterol- emia is an important risk factor of AD. Some studies also shown that the high cholesterol diet increased the accumulation of Aβand accelerate AD-related pathology. There was inseparable association between the APOAI gene polymorphism and plasma cholesterol level.MnSOD lied in the mitochondria. It was necessary superoxide dismutase for the body. It was a specific enzyme ,which clears superoxide radicals. It also had anti-inflammatory, anti-tumor, anti-aging effects, etc. Research shown that cell damage induced by free radicals is one of the pathomechanisms of AD.In our study, subjects were selected from ordinary middle-aged and elderly volunteers who were resided in the community of Shijiazhuang. We had extracted genomic DNA from leukocytes of volunteers's peripheral blood and measured serum biochemical indicators. MnSOD and APOAI genes were amplificated and digested by restriction according to literature. We took the general survey, MMSE and Chinese medicine dialectical type into consideration, then made a association analysis of genotype and blood biochemical indices and MMSE scores. This study provided clues for early prediction and assessment of AD risk factors.Method: We extracted genomic DNA from leukocytes of volunteers'peripheral blood and measured serum biochemical indicators after the relevant experimental test. We used phenol chloroform isoamyl alcohol for genome extraction. Polymerase chain reaction and restriction fragment length polymorphism were applied to the target-genes (MnSOD, APOAI). We applied the SAS V8 for statistical analysis. Non-parametric test was uesd for analysing the three aspects (biochemical parameters, genotyping and the tradition Chinese medicine syndrome state).Result:1. The gene of MnSODThere were 167 cases which had been successful amplificated and digested by restriction enzyme. Distributions of genotype and allele were accorded with Hardy-Weinberg equilibrium by applying chi-square test. Association analysis of Genotype and blood biochemical indices and MMSE , we obtained results as belows:For all male population, TG concentrations of AA genotype carriers were higher than AV genotype(P=0.006<0.05).TG concentrations of the carriers of A allele were higher than the carriers of V allele (P = 0.004 <0.05).For all male population, VLDL-Ch concentrations of AA genotype carriers were higher than AV genotype(P=0.019<0.05). VLDL-Ch concentrations of the carriers of A allele were higher than the carriers of V allele (P = 0.028<0.05).For less than 60-year-old male populations, TG concentrations of AA genotype carriers were higher than AV genotype(P=0.006<0.05). TG concentrations of the carriers of A allele were higher than the carriers of V allele (P = 0.017 <0.05).For Less than 60-year-old male populations, VLDL-Ch concentrations of the carriers of A allele were higher than the carriers of V allele (P = 0.022<0.05).2. The gene of APOAI-75bpThere are 179 cases had been successful amplificated and digested by restriction enzyme. Distributions of genotype and allele were accorded with Hardy-Weinberg equilibrium by applying chi-square test. Association analysis three of Genotype and blood biochemical indices and MMSE ,We obtained results as belows:For female populations, MMSE score of GG genotype carriers were higher than AA genotype(P=0.044<0.05) For more than 60-year-old female populations, MMSE score of the carriers of G allele were higher than the carriers of A allele (P = 0.046<0.05). For more than 60-year-old female populations, G concentrations of the carriers of G allele were higher than the carriers of A allele (P = 0.011<0.05).3. The gene of APOAI+83bpThere are 179 cases had been successful amplificated and digested by restriction enzyme. Distributions of genotype and allele were accorded with Hardy-Weinberg equilibrium by applying chi-square test. Association analysis three of Genotype and blood biochemical indices and MMSE ,We obtained results as belows:For all age all sex populations, TG concentrations of TC genotype carriers were higher than CC genotype(P=0.039<0.05).For all age all sex populations, G concentrations of TC genotype carriers were higher than CC genotype(P=0.037<0.05).For all age all sex populations , VLDL-Ch concentrations of TC genotype carriers were higher than CC genotype(P=0.013<0.05).For female populations, TCh concentrations of CC genotype carriers were higher than TC genotype(P=0.029<0.05).For female populations, MMSE score of TC genotype carriers were higher than CC genotype(P=0.04<0.05).For female populations, MMSE score of the carriers of T allele were higher than the carriers of A allele (P = 0.046<0.05).For less than 60-year-old female populations, TG concentrations of TC genotype carriers were higher than CC genotype(P=0.035<0.05).For less than 60-year-old female populations, TG concentrations of the carriers of T allele were higher than the carriers of C allele (P = 0.042<0.05). For more than 60-year-old female populations, TCh concentrations of CC genotype carriers were higher than TC genotype (P=0.005<0.05).For more than 60-year-old female populations, HDL-Ch concentrations of CC genotype carriers were higher than TC genotype (P=0.029<0.05).For more than 60-year-old female populations, TCh concentrations of the carriers of C allele were higher than the carriers of T allele (P = 0.006<0.05).For more than 60-year-old female populations, HDL-Ch concentrations of the carriers of C allele were higher than the carriers of T allele (P = 0.033<0.05).MMSE score of more than 60-year-old female populations: The carriers of CC genotype, which the median and quartile are as follows: 27 points, 3.00, while the carriers of TC genotype, which the median and quartile are as follows: 29 points, 2.00. Comparison of the two genotypes, we obtained: P=0.063.4. Blood biochemical parameters and the tradition Chinese medicine syndrome stateTCh concentrations: Yin-physical carriers were higher than Yang-physical carriers.TG concentrations: Yang-physical carriers were higher than Yin-physical carriers.Conclusion:There is a certain correlation by association analysis of APOAI and MnSOD gene polymorphism and blood biochemical indicator in residents of community of Shijiazhuang.There is a close relationship between concentration of TCh and the scores of MMSE, especially in the female of the polymorphisms of APOAI+83bp.