| Purpose: Along with the elevation of living standard and the change of eating habit in our country,the incidence of CAD is raising.So how to prevent CAD becomes to the important duty of medical personnel.Now CABG is one of the most utility means for treating CAD.From Favalora blested grafted GSV between AO and cabined coronary artery,CABG with GSV is becoming utility means for treating CAD in clinic.But the long-term restenosis rate is the graveness defect.So how to prevent restenosis is to become emphasis.The main agent of graft restenosis is the injury of blood vessel endothelium during vein collection,inflammatory cell infiltration, thrombosis.Further vascular smooth muscle cell migrations and enhancement, endothelial cell accrementition and confused,at last vein is arterialization. In other words, restenosis is a blood vessel repair react mediated by Inflammatory cell and inflammatory factor from injury which is created by touch and ischemia,and a remodeling course of blood vessel. It can be seen that inflammatory react is the most important key point.LTs is one of the most impotant inflammatory factors.The reaserch indicate that as endothelial injuries, mononuclear cell adheres to EC and start inflammatory react.EC expresses some adhesion molecules such as ICAM-1 and VCAM-1.These combine with homologus ligandβ1 andβ2 to educe effect. Friedrich's reaserch indicated that LTB4 can significant strengthen the affinity which ICAM-l and VCAM-1 combine withβ1 andβ2 ligand on mononuclear cell to precipitate adherence and start the inflammatory react of endothelium.LTB4 can significant strengthen proliferation and migration of VSMC.It is mainly mediated by I-κB kinase / NF2 B access.From this,LTs play a important role in inflammatory react after cell injury and remodeling by VSMC proliferation.So the prevention of nonage inflammatory react caused by injure is key point for protecting EC and control restenosis of CABG.Now there're many research of protecting step.Lots of these are which fluid to protect GSV.The most universal fluid is narceine fluid.The main effect of narceine is to expand blood vessel without any effect of restrainting inflammatory react.In view of this,the purpose of this topic is to search for a new protection path through compareing with narceine fluid and protection fluid which add to Pranlukast(one of CysLTRAs),for providing the new idea to improve restenosis.Methods: The experiment builds animal model with gafting rabbit external jugular vein to homonymy common carotid artery.Thirty healthy rabbit were divided stochastically to three group:A,B andC group.The external jugular vein which obtain in operation was flushed twice by 50mmHg pressure to leave with different protection fluid(heparin NS, narceine NS, narceine NS with Pranlukast)60 minutes.Part of these viens manufacture HE specimen and part manufacture transmission electron microscope specimen.The others used for building vien bypass.Two weeks later, to draw the materials from the middle of vien bypass made specimen. Transmission electron microscope observe ultrastructure of EC.HE staining and SP staining observe hyperplasia condition of vessel wall.And calculating proportion to contrast.Results: (1) Two rabbits were died during the experiment period.One is narceine group and another one is Pranlukast group.(2) The result of to comp- are thickening extent of living rabbits'vein bypass(L1/L2 %) manifest that:on- e-factor analysis of variance (F=50.16,P<0.05).To compare each other with L- SD statistical method :Pranlukast group(297.87±57.08) could control thicken- ing more than narceine group(478.88±46.61)or heparin group (480.13±27.46).(3)SP staining observed the ratio of generation cell number (%):one- factor analysis of variance (F=56.12,P<0.05).To compare each other with L- SD statistical method :Pranlukast group(31.23±3.50) could decreased generat- eon cell number more than narceine group(53.20±5.84)or heparin group (53.34±5.68). (4)TEM:To observation TEM specimen which soak for an hour of each group : Heparin group EC has damaged and become edema. We can s- ee mitochondria and ER edema,and part of organelle lost. The whole cells be- came loose. Intima is not continuous, or even interrupted, incomplete, cell co- nnectionns damaged, and the base connection is not closely. The layer of inti- ma attached platelets, cellulose,and part of layer of smooth muscle exposured. Narceine group EC and organelle became edema. The whole cells became loo- se but kept Integrity.layer of intima is more continuous than heaprin group but not smooth. We also can see that fraction attached platelets, cellulose. The eff- ect of Pranlukast group is better than both of heparin group and narceine group. The whole cells kept Integrity.The level of edema is lower. The connection of intima is close. Intima is continuous. To observation TEM specimen two wee- ks after graft of each group : Heparin group shows thicken- ing intima and en- dothelial cells increased. Organelles,such as mitochondria and ER,was genera- tion obviously.The intima of blood vessel attached lots of cellulose to lead not to observe clearly. Narceine group shows thickening intima and endothelial c- ells increased too. Organelles was generation.And the intima of blood vessel attached some cellulose. Pranlukast group shows the intima smooth.The EC and organelles increased and generation.Conclusions: (1) Pranlukast group can control restenosis of vein bypass more than narceine group or heparin group.Becouse it restrains inflammation react and protect integrality of EC better. Inflammation react is an important factor of restenosis.Narceine group and heparin group could't restrains to injure EC.(2)To compare TEM specimen which soak for an hour of each group shows that: The protection of Pranlukast group is best.The better is narceine group.The heparin group can't protect anymore.Because narceine can restraint spasm of blood vessel. Pranlukast group can restraint inflammatory reaction further.
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