| Objective To observe plasma fibrinogen level variation after acute spinal cord injury in rabbits model, and further the use of defibrase intervene. To explore the relationship between plasma fibrinogen levels and the expression of glial fibrillary acidic protein (GFAP) and vimentin(Vim) and the neurological function recovery after acute spinal cord injury. It provides new ideas for clinical treatment of spinal cord injury.Methods Sixty New Zealand white rabbits(male and female half and half) were divided randomly into saline intervention in the spinal cord injury group (A group) 20, defibrase intervention in the spinal cord injury group (B group) 20, non-spinal cord injury group (C group) 20. Laminectomy was implemented by posterior approach with fenestration on T8 vertebral level. Then the animals were made into models of acute spinal cord injury model by an impacting force of 10g×6cm according to the modified Allen's method. But the spinal cord were not been hit in C group. Post-operation managements included Penicillin200,000 u im qd in the first 3 days after surgery and artificial micturition until the automatic micturition recover. A group:5ml saline intraperitoneal; B group:10 units defibrase diluted with 5ml saline intraperitoneal; C group:5ml saline intraperitoneal. All animals were injected from postoperative 1 day once a day for 7 consecutive days. Blood specimens (ear vein) were collected for determination of fibrinogen at different time points before and after operation. The rabbits were evaluated the motor function as the advanced Tarlov method, spinal cord evoked protential, glial fibrillary acidic protein and vimentin immunohistochemical staining. The FIB concentration, advanced Tarlov score, latency and amplitude, positive cells of glial fibrillary acidic protein and vimentin expression were showed by way of average and standard deviation. The data was managed by the SPSS 16.0 software. The measurement data single effect of time factor was analyzed by repeated measurement of ANOVA. The measurement data effects between subjects were also analyzed by repeated measurement of ANOVA.The measurement data pairwise comparisons were analyzed by the method of LSD. The measurement data effect of two grouping factor was analyzed by independent-samples t-test or t'-test. The counting data effects between two groups were analyzed by Wilcoxon andMann-Whitney U.The counting data effects among three groups were analyzed by Kruskal-Wallis H. Results 1. There were different levels of plasma fibrinogen in A, B, C three groups before and after surgery, which was significantly higher than that preoperatively. The state continued to rise, reached a peak at the seventh day, and then began to decline to normal levels after 14 days. The comparison found that plasma fibrinogen levels on the third days were significantly different (P<0.05) between A and B groups or between B and C groups. Fibrinogen of B group was lower than that of A, C groups. The difference between A and B groups or between B and C groups was not statistically significant after 14 days (P>0.05). The difference between A and C groups was not statistically significant in each phase (P>0.05). 2. Kinematics scores and spinal cord evoked potentials showed that the difference was statistically significant (P<0.05) in the advanced Tarlov method, latency and amplitude before and after operation in A or B groups. The difference between A and B groups was statistically significant after the seventh day (P<0.05). The recovery in group B is high than in group A. 3. Glial fibrillary acidic protein and vimentin were markered by immunohistochemistry in A and B groups. The rate of positive cells in each phase between the two groups were statistically significant differences (P<0.05).Conclusion 1. There is a certain variation in plasma fibrinogen levels after trauma in animals, which may be a non-specific reaction of the body. 2. The use of defibrase can reduce plasma fibrinogen levels and promote the function recovering of spinal cord injury. Its mechanism might reduce the accumulation of fibrinogen in the spinal cord injury site and lower glial fibrillary acidic protein and vimentin expression in order to provide favorable conditions for recovery of neurological function.
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