IL-1? Promotes The Formation Of Glial Scar Via JAK2-STST3 Signaling Pathway At The Early Stage Of Spinal Cord Injury

Objective To investigate the effect of IL-1? on the expression of glial fibrillary acidic protein(GFAP)and vimentin in spinal cord injury and the role of JAK2-STAT3 signaling pathway in it.Methods(1)The experimental model of SCI was created by extradural compression of the spinal cord using an aneurysm clip,Rats were randomly divided into model group,sham operation group,IL-1? inhibitor IL-1RA group and IL-1? group according to different interventions,then were given normal saline,IL-1RA and IL-1? every 10?L respectively,sham group received only laminectomy.The levels of dyskinesia were measured by Basso Beattie Bresnahan(BBB)scores,and we used immunofluorescence assay,Western blot technique and immunohistochemistry technique to detect the expression of p-STAT3 and GFAP at corresponding time points(at the 8th hour,12 th hour,1st day,3rd day,7th day and 14 th day after SCI).The experimental model of SCI was created by extradural compression of the spinal cord using an aneurysm clip,Rats were randomly divided into model group,sham operation group,IL-1?+JAK2-STAT3 inhibitor AG490 group and IL-1? group according to different interventions.then were given normal saline,IL-1?+AG490 and IL-1? every 10 ul respectively,sham group recerved only laminectomy.The levels of dyskinesia were measured by Basso Beattie Bresnahan(BBB)scores,and we used immunofluorescence assay and Western blot technique to detect the expression of vimentin and GFAP at corresponding time points(at the 1st,3rd,7th and 14 th day after SCI).Results(1)At the 8th and 12 th hour after SCI,the model group expressed more p-STAT3 than the IL-1RA group(p<0.05),while expressed less p-STAT3 than the IL-1? group(p<0.05).The expression of p-STAT3 in each treatment group was significantly higher than that in sham group(p<0.01).At the 7th and 14 th day after SCI,the model group expressed more GFAP than the IL-1RA group(p<0.05),while expressed less GFAP than the IL-1? group(p<0.05).The expression of GFAP in each treatment group was significantly higher than that in sham group(p<0.01).At the 14 th day after SCI,the model group had higher BBB scores than the IL-1? group(p<0.05),while had lower BBB scores than the IL-1RA group(p<0.05).The BBB scores in each treatment group was significantly lower than that in sham group(p<0.01).At the 1st and 3rd day after SCI,there was no difference in the expression of GFAP between intervention groups and model group.At the 1st,3rd and 7th day after SCI,there was no difference in the BBB scores between intervention groups and model group(p>0.05).At the 7th and 14 th day after SCI,the model group expressed more vimentin and GFAP than the IL-1?+AG490 group(p<0.05),while expressed less vimentin and GFAP than the IL-1? group(p<0.05).The expression of vimentin and GFAP in each treatment group was significantly higher than that in sham group(p<0.01).At the 14 th day after SCI,the model group had higher BBB scores than the IL-1? group(p<0.05),while had lower BBB scores than the IL-1?+AG490 group(p<0.05).The BBB scores in each treatment group was significantly lower than that in sham group(p<0.01).At the 1st and 3rd day after SCI,there was no difference in the expression of GFAP and Vimentin between intervention groups and model group.At the 1st,3rd and 7th day after SCI,there was no difference in the expression of BBB scores between intervention groups and model group(p>0.05).Conclutions The secretion of IL-1? after acute spinal cord injury can promote the formation of glial scar around the injury location via the phosphrylation of JAK2-STAT3,which affects the rat hindlimb motor function.