Growth Inhibition By RASSF1A Gene And Relation With Cyclin D1 And P16 Gene In Gastric Carcinoma Cell Line SGC-7901

Objectives: In this study ,we investigated the effect of RASSF 1A gene on the grow -th of gastric carcinoma cell line of SGC-7901 and the correlation with cyclinD1,p16 gene in order to provide a theoretical basis for prevention and treatment of gastric carcinoma .Methods: The research objects were gastric carcinoma cell lines SGC-7901, pc DNA 3.1(+)SGC-7901, pc DNA 3.1(+)/RASSF1A-SGC-7901. The proliferation and apoptosis of cells were detectd by MTT assay and flow cytometry ,respectively . RT-PCR was applied to detect the expression of cyclin D1 gene and p16 gene in the three kinds of cell lines.Results:Compared with SGC-7901 and pc DNA 3.1(+)SGC-7901,the results of MTT showed that the cell proliferation of pc DNA 3.1( + )/RASSF1A -SGC7901 was inhibited ,and the difference was significant (P<0.05) ,and the rate of inhibition among one week were 0%,14.6%,22.0%,29.2%,31.6%,33.3% and 44.2%. Flow cytometry assay showed the apoptosis rate in pc DNA 3.1 (+ )/RASSF1A-SGC -7901 went up significantly and the cell cycle was changed obviously. The numb- er of G0/G1 stage cells increased and the number of S stage cells was decreased (P <0.05). RT-PCR results demonstrated that the expression of cyclin D1 gene was decreased and the expression of p16 gene was increased in pc DNA 3.1 (+)/ RASSF1A– SGC-7901 cells ,and the difference were siginificant(P <0.05) .Conclusions : 1,RASSF1A gene can block the G0/G1 phase of cell, promote the cell apoptosis in human gastric cancer cell SGC-7901.2,cyclin D1 and p16 involve in growth inhibition by RASSF1A gene in SGC-7 901 . RASSF1A can inhibit the expression of cyclin D1 gene and up-regulate the expression of p16 gene.