The Establishment Of Golen Hamster Cheek Mucose Carcinom A Related Gene Differential Expression Profile And Bioinformatics Analysis

Oral and Maxillofacial Cancer is recognized as the 6th most dangerous malignant tumor in the body and increases year by year. 90% of Oral and Maxillofacial Cancer is OSCC. Great progess has been made in the treatment of OSCC. However, the mechanism of the development and pathological changes of OSCC have not been fully understood, which prevents major changes in the clinical curative effect. It is acknowledged that the development of cancer is a multi-gene and multi-step continuous dynamic process. To this day, it has been proved through clinical and concerned research that the initial oral mucosa carcinogenesis is caused by Mucosa Epithelial dysplasia of the normal oral mucosa, the consecutive dynamic changes in oral squamous cell carcinoma (OSCC) involving multi-gene and multi-path transformation. However, no research report on the screening of major pathogenic gene or signal pathway related to oral mucosa carcinogenesis from complete genome level has been found.This research establishes a model of carcinogenesis of hamster buccal-pouch mucosa by inducing DMBA to Syrian hamster buccal-pouch mucosa and analyzing the differentially expressed genes of the carcinogenesis of hamster buccal-pouch mucosa by the use of cDNA microarray involving 41,000 lines of genes/Agilent of ESTs. Consequently, 1,943 lines of differentially expressed genes are found and through GO functional classification analysis, these differentially expressed genes are revealed to be related to apoptosis and tumor-related genes, structural elements related genes, metabolism related genes, signal transduction related genes and other 6 types of genes. Pathway analysis has detected obvious changes in 27 pathways which are related to only 136 of 1,943 differentially expressed genes mentioned above. We consider that this 136 differentially expressed genes are important candidate genes, which ultimately lead to cancer of buccal mucosa. in this 136 important candidate genes, 13 genes enriched in more than three pathways.Therefore, it is believed that the above-mentioned 13 lines of genes are the major ones for the carcinogenesis of hamster buccal-pouch mucosa and the in-depth study on them will provide effective means for revealing the molecular mechanism of oral mucosa carcinogenesis and identifying the targeted therapy.