Expression Of MIR-21 In Breast Carcinoma And Its Clinical Significance

Objective:To examine the level of expression of microRNA-21 in breast cancer and normal tissues more than 5 cm away from the tumor tissues , The association of miR-21 expression with the clinical pathological factors of breast cancer were analyzed .Methods:Specimens of breast cancer and normal tissues more than 5cm away from the tumor tissues were collected from 60 patients, The miR-21 expression was detected by Stem-loop real-time RT-PCR . Levels of miR-21 expression by tumor size, TNM clinical stage, histology, lymph node involvement, hormone receptor status were compared .Results:High sensitivity, specificity and precision of Stem-loop real-time RT-PCR allows for direct analysis of expression of miR-21 of breast cancer . With normal tissues, obvious high level of expression of miR-21 was detected (P < 0.001 ),Elevated miR-21 expression was also positively correlated with Advanced TNM stage, lymph node metastasis, and low PR levels(P =0.007,0.001,and 0.012 respectively), Moreover, We discover for first time ,levels of expression of miR-21 in triple-negative breast cancer(TNBC)is high than other types(P =0.029).Conclusion:High levels of expression of miR-21 are correlated with malignant degree of breast cancer.