Electrospinning Nanofiber Of β-TRICAlCIUM Phosphate/gelatin Biomimetics For Guided Tissue Regeneration Membrane

AIM: Preparing a kind of gelatin(Gel) withβ-tricalcium phosphate(β-TCP) hybrid nanofibrous membrane and testing its biocompatibility for guided tissue regeneration.METHODS: The hybrid nanofibrous membrane is made by the electrospinning technique. Through the ways of scanning electron microscopy (SEM), mechanical tensile test, MTT test and cell co-culture with the material to character the material and study its biocompatibility.RESULTS: After crossing-linking the average diameter of hybrid nanofibrous were increased from 200-300nm to 500-600nm. By cross-linking its tensile fracture stress is markedly improved from (1.08±0.24)MPa to (6.47±0.85)MPa. In regard to cytotoxicity there is significant difference between the positive control and the hybrid nanofibrous membrane(P<0.01),and there is no significant difference compared with the negative control.At last, the osteoblast-like cells(MG-63) could successfully attach and proliferate on the hybrid nanofibrous membrane.CONCLUSION: Theβ-tricalcium phosphate / gelatin guided tissue regeneration membrane can be prepared by the electrospinning technique successfully. Because of its good biocompatibility, the hybrid nanofibrous membrane can be used for tissue engineering, indicating that the hybrid nanofibrous membrane is a promising material for periodontal tissue regeneration.