| Panax notoginseng is one of traditional Chinese herbal medicines in Guangxi. The main active ingredient in it of ginsenoside Rgl is one saponin in panax notoginseng has obvious effect in anti-coagulation and platelet aggregation,so it can be used as a kind of drug in treating cardia-cerebrovascular disease. The ethanol extraction method is used to extract from panax notoginseng of Guangxi, and then through the macroporous resin for enrichment and purification of ginsenoside Rgl, and then make monomer ginsenoside Rgl by centrifugal thin layer chromatography, then study the method about how to making the Rg1 liposome and evaluate its quality and stability.Content and results as follows:1.Through the comparison of the backflow method, ultrasonic and immersion method, microwave extraction, We can get the conclution backflow is the best method for extraction, which can obtain 7.88%. Extraction method is: 70% ethanol solvent, dosage is 8 times of medicinal, reflux extraction 2 times in 80℃, we need to do it twice total. In the end we can get 25.8% of Dry paste, 13.37 percent Rg1 saponins in it. Thus, we can calculates the extraction rate of ginseng saponins Rg1 saponins is 3.45%, and recovery rate of extraction is 95.3% by the backflow method.2.With D-101 macroporous resin preliminarily preliminarily separate the dried paste of panax notoginseng the extract, and collect the high purity analytical solution of ginseng saponins Rg1 by TLC qualitative test , and emerge and evaporate them, and we get the rate of coarse product purity is 56.7%.Through comparing the static absorption rate and analysis rate of the different types of macroporous resin, we screening and get the most optimal resin type.At the same time,, establishing the methods of aldehydes - high chlorine acid colorimetric determination of total ginsenosides panax notoginseng total saponins when panax notoginseng total saponins is defined as the measurement index of the eluent.We optimize the separate condition by testing the elution rate of panax notoginseng total saponins , and get the optimum technological conditions of macroporous enrich and purificate Rg1 is: D - 101 type macroporous, the concentration of sample is 30mg/mL, the volume is 10mL, velocity is 0.5 mL/min, elution is 70% ethanol, which volume is 120mL , velocity is1.0 mL/min.3.Simple Process ginseng saponins R1 by Centrifugal chromatography and collect high purity of tube to merger, afer the second separation, crystallizate them again, and we get the purity of 91.3% saponins ginseng Rg1, what we do above is TCL testing. Through the comparison of the stationary prescription , type of elution,the proportion of elution , which are the conditions of chromatographic, we get the best conditions are: using silica gel HF254 gypsum which absorbent layer thickness is 2mm as stationary phase, the proportion of eluent is CH2Cl2∶MeOH = 7∶1.the volumn of simple is 500mg, concentration is 125mg/mL, velocity of eluent is 4mL/min.4.Making the ginseng Rg1 into liposomes by film ultrasound and scattered.and get the best prescriptionfactor of making Rg1 liposomes is : the quality rate of soy lecithin and cholesterol is 3∶1, physiological saline as water medium, the quality rate of lipid and Rg1 is 10∶1, the concentration of Ginseng saponins Rg1 which are in the physiological saline are 2mg/ml , and establish the method of HPLC determinate the content of Ginseng saponins Rg1 .Through the determination, the Sealed rate of the Rg1 liposome which we make is 2.482% , drug loadings rate is 2.482%. Study for the leakage rate,long-term stability and vitro release performance of the liposomes of plasma, and analyse the reason of low sealed rate, in addition, we try to improve to preparation technology in order to increase the sealed rate.
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