Distribution And Postmortem Redistribution Of MDMA In Rats

Objective To study distribution of MDMA in acute poisoned rats. To explore postmortem redistribution of MDMA in rats and the influence of temperature to postmortem redistribution. Methods (1) MDMA solutions were infused in the stomach of rats animal model and rats were killed 2 h later. Specimens ( including cardiac blood,cardiac muscle,liver,spleen ,lungs,kidney,brain) were extracted by the ether and derivatized with trifluoroacetic. A method for the determination of the level of MDMA in tissues and blood of rats was developed using gas chromatography/mass spectrumetry(GC/MS) and selected ion monitoring (SIM). (2) After instillation in the stomach and sacrifice,forty-two rats left at ambient temperature of either 17℃or 4℃.At 0,2,24 and 48h postmortem,samples including cardiac blood,femoral blood,cardiac muscle,liver,spleen and lungs were taken and extracted with liquid-liquid and derivation. MDMA levels were detected by gas chromatography with nitrogen phosphorus detector. Results (1)The concentration of MDMA in tissues was higher than in blood,there was significant differences. The order of concentration of MDMA in acute poisoned rats is as follow: spleen >stomach >lungs >liver >kidney >brain >cardiac muscle >cardiac blood. (2) With increasing postmortem interval, MDMA concentrations in cardiac blood and spleen show a rise tendency. MDMA level in lungs show a decline tendency. The liver MDMA level descended after going up first and the myocardial MDMA level had no obvious variety. (3) MDMA level of tissues at 17℃and 4℃were compared and there was not statistically significant(P > 0.05). MDMA level of blood at two distinct ambient temperatures 2h postmortem were compared and there was not statistically significant(P > 0.05). But at either 24h or 48h postmortem, MDMA level in cardiac blood at two temperatures were compared and there was significant differences(P <0.01). Conclusion (1)Our data demonstrates that MDMA were distribute abroad in tissues and blood, and it may damage these tissues. (2) These results have proved the presence of MDMA postmortem redistribution in rats. (3) There was different influence of temperature to blood and tissues at MDMA postmortem redistribution. MDMA blood concentration at postmortem was more stabile at low temperature.