Study On The Establishment Of VILI Model And The Expression Of IL-1β And NF-κB P65 After High Tidal Volume Ventilation In Rabbits

Part I Application of high tidal volume ventilation to construct the best VILIexperimental animal model of rabbits.Objective: To employ high tidal volume ventilation to construct the best VILI experimental animal model of rabbits.Methods: According to the principal of Fibonacci's method, we designed three different ventilatory capacity(68mL/kg, 60mL/kg and 45mL/kg) into rabbits keeping on venting for one minute induced acute lung injury, observed in different time (Oh, 12h, 24h, 48h respectively) after ventilation, added up survival numbers of rabbits to calculated their survival rates, calculated lung W/D to reflect edema of lung, HE staining to reflect the changes of lung histomorphology.Results: 1 , the survival rates of group A,B, C(V_T68mL/kg,60mL/kg and 45mL/kg respctively) were 58.33% (7/12×91.67% (11/12) and 100% (12/12) respectively; 2, to compare with normal control group (4.40 ± 0.02 ), lung W/D of group A,B,C (V_T68mL/kg, 60mL/kg and 45mL/kg)were not marked change at 0 hour, the relative values were 4.46±0.08, 4.48±0.08 and 4.43±0.12 respectively; obviously increased at 12 hours, the relative values were 5.27±0.18,4.84±0.12 and 4.64±0.06; reached peak at 24 hours, the relative values were 5.50±0.18,5.29±0.12 and 4.88±0.07; sequent decreased at 48 hours, the relative values were 5.00±0.31, 4.77±0.21 and 4.46±0.10; 3, HE staining showed that the changes of lung tissues were extremely obvious, obvious, not obvious in the group A, B, C(V_T68mL/kg, V_T 60mL/kg and V_t 45mL/kg) respectively.Conclusion: The best VILI experimental animal model of rabbits was established successfully after high tidal volume ventilation , which indexes were tidal volume(VT) of 60mL/kg, respiratory rate (RR) of 50 breaths per minute and inspiratory oxygen fraction(FiO₂) of 21%.