Effects Of Folic Acid And Exercise On Blood Lipid Levels And The Antioxidant Capacity Of Homocysteine Rats

Purpose:Investigate effects of folic acid and exercise on blood lipid levels and the antioxidant capacity of homocysteine rats, make a discussion to exercise combined with folic acid on HHcy rats with Hcy, folate, lipid levels and antioxidant ability, to prevent the development of AS to provide experimental basis.Method:The male SD rats are randomly divided into the normal food matched Control (C), normal diet and exercise group (CT), methionine diet control group (M), methionine diet and exercise group (MT), methionine diet+ folic acid group (MF), methionine diet+folic acid+ exercise group (MTF),-10 in each group. Methionine group of rats fed a 3% ratio by adding L-methionine into methionine feed. CT, MT, MTF free weight bearing group were swimming 6 days a week for 8 weeks. The first day of the exercise the first week of 10min, increasing each day, to the second week to 90min and maintained. MF and MTF fed a dose of 20μg/kg of folic acid. Slaughtered measurement of plasma Hcy, serum TG, TC, HDL, LDL, ApoA, ApoB, folic acid, plasma SOD, MDA, GSH, liver SOD, MDA, GSH content. All data processed in SPSS 13.0 to calculate the average (X) and standard deviation (SD), comparison between groups using single factor analysis of variance, P<0.05 as the significance level, P<0.01 as very significant level.Result:①methionine diet control group (M) and the normal food matched Control(C) comparison, Hcy, TG, TC, LDL, ApoB concentration, TG/HDL, TC/HDL, ApoB/ApoA, ApoB/HDL, LDL/HDL concentration, plasma and liver MDA concentration, liver SOD,GSH reduced;②Methionine diet and exercise group (MT) and methionine diet control group (M) comparison, Hcy, TG, LDL concentration decreased, TG/HDL, TC/HDL, ApoB/ApoA, ApoB/HDL, LDL/HDL ratio decreased, plasma and liver SOD increased, MDA reduced;③Methionine diet+folic acid group (MF) and methionine diet control group (M) comparison, Hcy, LDL, ApoB concentration decreased, HDL, ApoA concentration, TG/HDL, TC/HDL, Apo -B/ApoA, ApoB/HDL, LDL/HDL ratio decreased, plasma and liver SOD, GSH increased, MDA reduced;④Methionine diet+folic acid+exercise group (MTF) and methionine diet control group (M) comparison, Hcy, folic acid, TC, LDL, ApoB concentration decreased, TG/HDL, TC/HDL, ApoB/ApoA, ApoB/HDL, LDL/HDL ratio decreased liver SOD, GSH increased plasma and liver MDA reduced; methionine diet+folic acid+exercise group (MTF) and methionine diet and exercise group (MT) Comparison of plasma and liver MDA decreased plasma SOD decreased liver GSH increase; methionine diet +folic acid+exercise group (MTF) and methionine diet+folic acid group (MF) compared to plasma GSH decrease, MDA in plasma and liver were significantly reduced.Conclusion:High-methionine diet in rats could increase Hcy levels, produce homocysteine (HHcy), and to homocysteine blood lipid metabolism disorder, decreased antioxidant capacity. The right amount of aerobic exercise and/or appropriate folic acid supplementation can reduce homocysteine methionine diet-induced hyperlipidemia rats Hcy levels, improve blood lipid metabolism and reduce oxidative stress levels, increase their antioxidant capacity; The same time the two interventions is not very different and individual intervention, only in raising the level of the body of folic acid in combination is more effective.