Effect Of Overtraining On Intestinal Immune Function In Rats And Intervention Of Glutamine Or Soy Protein Isolate Supplementation

Objective:To establish rat model for overtraining and explore the effect of overtraining on intestinal immune function in rats and its mechanisms. At the same time of exercise training, glutamine or soybean peptide was supplemented, to explore the intervention effect of glutamine or soybean peptide on the intestinal immune function in overtraining rats and its mechanism.Method:Experimental rats were randomly divided into control group, overtraining group, glutamine intervention group and soybean peptide intervention group. The rats in overtraining group were carrying on exhaustive training for 9 weeks, 6 times/week. The rats in glutamine intervention group or soybean peptide intervention group were supplemented Gln with 1.5g /kgBW dosage or soybean multi-peptide with 500mg/kgBW dosage before heavy training. 9 weeks later, SOD, MDA, Gln, the sIgA content and CD4~+, CD8~+ quantity in small intestine and T, C, Hb, WBC content in blood were examined.Results:â‘ Compared with the control group, the serum testosterone concentration, the T/C ratio obviously reduced, the serum cortisone concentration obviously increased; Hb, WBC quantity in blood obviously reduced in overtraining group. The supplement of Gln might obviously obviously increased Hb content, serum testosterone concentration and the T/C ratio, reduce serum cortisone concentration Obviously. The supplement of SPI might obviously increase Hb content, serum testosterone concentration and the T/C ratio.â‘¡Compared with the control group, in the small intestine tissue of overtraining group, sIgA content, CD4~+ quantity and the CD4~+/CD8~+ ratio obviously dereased, CD8~+ quantity obviously increased. And SOD activity, Gln content obviously decreased, MDA content obviously increased.â‘¢Compared with the overtraining group, in the small intestine of glutamine intervention group, sIgA content, CD4~+ quantity, CD4~+/CD8~+ ratio increased significantly, CD8~+ quantity obviously reduced. Although SOD activity was no significant change, MDA content in the small intestine obviously reduced.â‘£Compared with the overtraining group, in the small intestine of soybean peptide intervention group, sIgA content was no significant change, but CD4~+ quantity, CD4~+/CD8~+ ratio increased significantly, CD8~+ quantity obviously reduced. And SOD activity increased, MDA content in the small intestine reduced obviously.Conclusion:â‘ Establishing of the overtraining modle in this research was successful. Supplementing Gln or SPI in the training process might prevent the occurrence of overtraining.â‘¡Overtraining might cause immunologic function in small intestinal tract of rats to reduce obviously. In the small intestinal tissue of rats with overtraining, the significant decrease of Gln concentration and increase of metablism of free radical, might be main mechanism that overtraining causes immunologic function in the intestinal tract to reduce.â‘¢Supplement of Gln could improve immunologic function in small intestinal tract of rats with long-term heavyload training. Gln supplement might prevent Gln content to decraese and MDA content to increase in the small intestine of rats with heavyload training, it might be the main intervention mechanism which Gln supplement prevented the decrease of immunologic function in the intestinal tract.â‘£Supplement of SPI could improve immunologic function in small intestinal tract of rats with long-term heavyload training. SPI supplement might prevent the decrease of SOD activity and the increase of MDA content in the small intestine tissue of rats with heavyload training, it might be the main intervention mechanism which SPI supplement prevented the decrease of immunologic function in the intestinal tract.