| Objective:Ulcerative colitis (UC) is a chronic, nonspecific inflammation in the rectum and colon disease. Stop when illness weight ranges, while, in delay again and again, the episode and remission of appear alternately, on the basis of clinical characteristic, clinical types in chronic recurrence, the most common type of ulcerative colitis.There is a certain tendency of cancerous and ulcerative colitis.Current research on the development of ulcerative colitis is not entirely clear, diagnosis and differential diagnosis, illness evaluation aspects of UC has shortcomings, so did not have a great breakthrough on therapy.And the treatment of traditional Chinese medicine for ulcerative colitis has its unique advantages, but at the moment of UC diagnosis and treatment based on syndrome differentiation of traditional Chinese medicine lack of unified standard, it’s not clear Chinese medicine targets, there is no objective and quantitative laboratory indicators to achieve individual diagnosis and treatment standardization and scientization, therefore in the treatment of progress is slow.This study aims to through the application of metabonomics research techniques to explore potential biomarkers of ulcerative colitis, to explain the etiology and pathogenesis of UC provide objective laboratory indexes;Based on the virtual empirical ulcerative colitis patients plasma metabonomics studies, reveals the actual situation of syndrome differentiation characteristics of metabonomics, to explore evidence-based medicine of traditional Chinese medicine to provide new research ideas.This thesis is divided into three parts:the first part is the overview section, divided into traditional Chinese medicine and Western medicine Summary Summary, and summarized and advanced in our knowledge about the disease,; the first paper summarized TCM disease diagnosis of ulcerative colitis associated evolution and category, etiology and pathogenesis, syndrome classification and treatment of such research;The article elaborated the modern medicine research progress of ulcerative colitis, focus on the etiology and pathogenesis, related molecular mechanisms are reviewed and metabolomics research progress about the disease.The second part is clinical trials, according to the clinical cases were collected for general survey statistics, using 1H-NMR metabolomics technology is the study of the characteristic metabolites of chronic recurrent Ulcerative colitis.The third part for ulcerative colitis deficiency and excess plasma metabolomics research, aims to find differences metabolites revealed the empirical characteristics of metabonomics.Methods:1 Collect 31 patients with ulcerative colitis and 40 cases of healthy controls of blood and urine.’H-NMR technique wasused to obtain the brain metabolism,the data were normalized, integrated and treated by SIMCA-P software pack to analize the major component (PCA) and the partialleastquares discriminate (PLS-DA) in combination with OSC method, in this way, thecorresponding score scattering plot and loading plot were obtained, Thus each sample metabolites of visual information and find out the metabolic biomarker.231 patient were divided into two groups:Plasma samples of 17 uc patients with excess syndrome and 9 uc patients with deficiency syndrome. Using the same analysis method in view of the two groups of plasma metabonomics analysis, find out the differences between material change trend in two groups.Result:1 Of the study of 31 cases,12 cases women and men (19 cases), minimum age 19, the biggest age 80 years, mean age was 42.9±15.5 years old.2 The study found that exist in the patients with 31 cases of modern abnormal cases, a total of 27 cases, accounting for 87.1% of the total number of cases, including irritability, anxiety, worry and mental tension, the largest proportion of 74.2%,45.2% and 48.4%, respectively,3 This in 31 cases, caused and aggravating factor of UC to climate change and food section does not see more,38% and 30% respectively, followed by 24% of modern not free.In the most common main catch cold catch cold in winter in climate change.4 Effect is the main symptom of life in patients with mucous bloody stool and diarrhea, each accounted for 35.5% and 35.5%.5 Chronic recurrent ulcerative colitis deficiency and excess traditional Chinese medicine, syndrome of dampness-heat is given priority to partial empirical a partial deficiency syndrome of spleen.6 For chronic recurrence in patients with ulcerative colitis and healthy controls (plasma and urine metabonomics research found that:compared with healthy controls, patients with UC acetyl acetate, acetone content in plasma increased significantly, lactic acid, anserine, alanine and isoleucine content decreased obviously.And urine creatinine, glycerate, isocitrate content increased significantly, hippuric acid levels drop. Statistically significant difference (P < 0.05).7 In view of the deficiency of traditional Chinese medicine plasma metabonomics deficiency and excess study found that:glucose, threonine, carnosine in partial excess syndrome than high content in partial deficiency syndrome;And valine, alanine, glycine in partial empirical than low content of partial deficiency syndrome.Conclusion:1 Chronic recurrent ulcerative colitis average age was 42.9±15.5 years, with the average Chinese patients with UC diagnosis was 40.6 years age difference is not big.Among them 18 to 40 patients more, with the onset of epidemiological investigation age tend to occur in the 20-40 years old are consistent.2 Of modern changes in the development of UC emphatically with the role, and climate change, and eating can induce or aggravate the disease, the patient should be kept in a buoyant mood in life, pay attention to cold warm and regular meals.The above factors should be considered in the clinical applying pesticide.3 The main symptom of chronic recurrent ulcerative colitis is mucous bloody stool and diarrhea.4 UC patients plasma and urine of metabolic spectrum deviate from healthy people, acetyl acetate, acetone, lactic acid, anserine, alanine and isoleucine, isocitrate, hippuric acid substances such as involved in the development of UC, could serve as molecular markers in early diagnosis of UC.Further illustrate the occurrence/development of type chronic recurrent ulcerative colitis and sugar metabolism, energy metabolism and lipid metabolism disorders.5 Alanine, glycine, valine glucose, threonine, anserine and other metabolites could be the difference between ulcerative colitis deficiency and excess potential biomarkers.Also further illustrate type chronic recurrent ulcerative colitis virtual demonstration may be associated with sugar, energy, amino acid metabolic disorders.Moreover through the study of the disease metabonomics urine sample, found that the results compared with healthy controls, there is no significant separation, and in the third part is used to identify the false positive urine metabonomics model analysis results meaningless.Considering the urine metabolites may be greatly influenced by external factors, urine is not recommended for 1H-NMR metabolomics research. |
PDF Full Text Request