Expression Of Long - Chain Non - Coding RNA In Embryonic Rhabdomyosarcoma And Acute T Lymphoblastic Leukemia

Objective:In the early stage of our study, we found a new Long non-coding RNA (LncRNA), which named T-ALL-R-LncR1. Moreover, further research proved that T-ALL-R-LncRl was associated with malignant tumors, and could promote the tumor cell growth. In order to search a new strategy for the treatment of embryonal rhabdomyosarcoma (ERMS), we detected the expression of the long non-coding RNA T-ALL-R-LncR1 in RD cells and tumor tissue of ERMS mouse model, to expand a new direction for the treatment of ERMS.Methods:RD cells were Cultured in vitro, cells in the logarithmic growth phase were obtained the lived. RD cells in logarithmic growth phase were made into single cell suspension, the suspension was injected subcutaneously into the BALB/c nude mice to establish the ERMS mouse model. After cell inoculation, tumor growth were observed and recorded. The nude mice were sacrificed 4 weeks after tumorigenicity and the tumor blocks were took out. The pathological features were observed through the HE staining, simultaneously the protein expression of Desmin, Myogenin, MyoD1 and Myoglobin were detected through immunohistochemistry, the Pathological type was judged. The expression of T-ALL-R-LncR1 was detected by RT-PCR in RD cells and the tumor tissue obtained from the ERMS mouse model.Results:The RD cells were cultured in vivo successfully, T-ALL-R-LncR1 was found in RD cells determined upon RT-PCR and sequencing. Solid tumors were obviously seen in all the nude mice 1 week after cell implantation, and the tumor forming rate was 100%. The tumor tissues were observed through HE staining and immunohistochemistry during microscopic, the pathology classification of the tumor tissue was confirmed ERMS. The T-ALL-R-LncRl was also found by RT-PCR in all of the tumors which were took from the ERMS mouse model.Conclusion:The T-ALL-R-LncR1 could be found not only in RD cells but also in tumor tissue got from the ERMS mouse model. The ERMS mouse model provides a reliable experimental model. These results may be very significant to understand the molecular mechanism, diagnosis and treatment in ERMS.Objective:To analyze the expression profile variation of long non-coding RNA(LncRNA) in normal human peripheral blood mononuclear cell(PBMC) and normalized human T-cell acute lymphoblastic leukemia(T-ALL) cell line (Jurkat cell line).Methods:Jurkat cell line was cultured in vitro. The normal human peripheral blood mononuclear cell (PBMC) was separated with Ficoll lymphocyte separation Liquid. Total RNAs of the two kinds of cells were extracted by TRIzol, followed by RNA quantification and quality control. The LncRNA microarray technology was used to establish the differentially expressed spectrumand and the bioinformatics analysis was used to find the useful LncRNA for further study of T-ALL.Results:There are significantly differences in LncRNA between Jurkat cell and PBMC. We totally detected 27086 LncRNAs, and there were 11797 LncRNAs had been changed from Jurkat cell to normal contorl.4709 LncRNAs were upregulated more than 2 times, which included 223 LncRNAs upregulated more than 10 times; and 7089 LncRNAs downregulated more than 2 times, included 764 LncRNAs downregulated more than 10 times.Conclusion:There was a obvious LncRNA expression difference between Jurkat celld and normal human PBMC. Used bioinformatics analysis, we found some LncRNAs which may had important biological function, they provided an important basis for further study of the disease occurrence and regulation in T-ALL.