Bioinformatics Analysis And Expression Of PPIL6 In Children With Acute Lymphoblastic Leukemia

Background:Acute lymphoblastic leukemia is the most common malignant tumor of children and young people in China, accounting for 70% of childhood leukemia. In recent years, with the progress of treatment of leukemia, the complete remission rate and long-term disease-free survival rate of ALL children has been significantly improved. But the therapeutic effects were not satisfied for a small number of children. The insensitive to chemotherapy and recurrence may be the main reasons of poor therapeutic effect, and its molecular mechanism is still unclear. Recent researches have found that the conversion of oncogenes and aberration of tumor-suppressing genes play an important role in the carcinogenesis and development of ALL. So further study of oncogenes or tumor-suppressing genes can help to find new ways to improve the prognosis of ALL. Kang rui et al. has found that a gene in 6q21 named PPIL6 may be a new candidate tumor suppressor gene. Therefore, further study of the relation between PPIL6 expression and ALL may help to filter a new tumor suppressor gene, which can be useful for early diagnosis, monitoring and prognosis of ALL, thus providing new ideas for gene-targeted therapy with ALL Objective:To make bioinformatics analysis of PPIL6 gene based on the human genome database, which helps to provide theoretical basis for further study of PPIL6 gene function and helps to choose animal models for PPIL6 gene cloning. To investigate the role of the expression of PPIL6 gene in the occurrence and development of ALLMethods:According to the PPIL6 gene sequences logged in GenBank,we used the bioinformatics sofeware to analysis biological information of PPIL6 gene.By the methods of RT-PCR, the expressions of PPIL6 in children with ALL(including 20 patients of untreated,16 cases of remission,10 patients of relapsed) and control group(14 patients with anemia of unknown) were detected in the bone marrow mononuclear cells.Results:PPIL6 gene was located in a genomic sequence named NC_ 001111298 in 6q21, and its cDNA length was 4223 bp. The sequences of PPIL6 gene family was highly conseverd in human and eight other species. The protein encoded by PPIL6 gene was a kind of alkaline protein, consisted of 337 amino acids, the fomular of which was C1722H2658N474O495S12. The coding products of PPIL6 were widely distributed in cell membrane, nucleus and various cell organelles. The expression of PPIL6 were significantly lower in untreated ALL group and relapsed ALL group (0.25±0.19,0.32±0.15) than that in the control group (0.55±0.33),p<0.05. The expression of PPIL6 in remission ALL group was higher than that in untreated group(0.51±0.33 VS 0.25±0.19), p<0.05. The expression of PPIL6 in remission group was higher than that of the relapse group, but the difference was not significant (p>0.05). The difference of PPIL6 expressions between the control and remission groups was not significant (p>0.05).Conclusions:Bioinformatics analysis provided evidences for PPIL6 gene cloning and further study of the functions of PPIL6 gene. The study of the expressions of PPIL6 in ALL groups and control group revealed that PPIL6 gene may be associated with the disease activities of children with acute lymphoblastic leukemia.