Study On The Efficacy And Mechanism Of Jiejiugan Prescription In Treating Alcoholic Fatty Liver

Purpose:In this study, the rat model of alcoholic fatty liver was prepared to research the role of Jiejiu Baogan Decoction played in treating alcoholic fatty liver and to explore its mechanism for providing a theoretical basis for the Jiejiu Baogan Decoction in the treatment of alcoholic fatty liver and providing new ideas and more choices for the treatment of alcoholic fatty liver.Material and method:After adaptive feeding for a week, 55 Healthy female Wistar rats of SPF, were randomly divided into 5 groups : control group,low dosage group of Jiejiu Baogan Decoction(low dose group), middle dosage group of Jiejiu Baogan Decoction(middle dose group), high dose group dosage group of Jiejiu Baogan Decoction(high dose group), model group. Each group contains 11 rats. The control group received standard diet. In order to replicate alcoholic fatty liver rat model, other groups are to be 56 degrees of liquor 10 m L / kg orally twice daily interval 6h, 6 weeks, combined with high-fat diet.After the modeling, the model group and the control group were given normal saline 10 ml / kg body weight orally; low, medium and high dose groups were given equal volume of concentrated liquid of Jiejiu Baogan Decoction in accordance with 12.5g/kg·d、25g/kg·d、37.5g/kg·d were fed once a day for 4 weeks. All rats received 10 weekend anesthesia, abdominal aortic blood, liver rapidly removed, weighed to calculate the liver index, HE staining of liver pathology, automatic biochemical analyzer, including serum ALT(alanine aminotransferase), AST(aspartate aminotransferase), CHOL(cholesterol), TG(triglyceride) levels. Determination immunohistochemistry liver tissue NF-κB(nuclear factor-κB), ICAM-1(intercellular adhesion molecule-1) expression.Results:1. Compared with the blank control group, model group, Jiejiu Baogan Decoction every dose group appeared different degrees of fatty degeneration and inflammation, which in the model group is most serious, compared with the model group, Jiejiu Baogan Decoction in each dose group of lipid degeneration and inflammatory activity is significantly reduced. 2. The expression of the liver index, TG, CHOL, ALT, AST and NF-kappa B, ICAM-1 of the rats in the model group was higher than the blank control group, while the body weight was significantly lower than the control group. 3. Jiejiu Baogan Decoction each dose group rat liver index, TG, ALT, AST compared with the model group have different degrees of decline, in which the most significant decline in the high-dose group; the high-dose group NF-κB, ICAM-1 expression than model group decreased significantly, CHOL high dose group was significantly lower than the model group.Conclusion:1. Jiejiu Baogan Decoction decoction can relieve alcoholic fatty liver in rats liver pathological changes, reduce blood fat, improve liver function, has curative effect on alcoholic fatty liver in rats. 2. Liver hangover party may regulate NF-κB, ICAM-1 expression in this pathway play a role.