Effects Of Immune Activation On The Surface Antigen Of DC-CIK Membrane In

Objective:The DC-CIK cells which are the first choice of the anti-tumor adoptive immunotherapy can be amplificated hundreds of times in vitro. However, the clinical effect is still not good enough. It is all because that we all focus on the amplification in vitro but ignoring the inside activation. The ancient Huangdineijing says that the five visceral organs are interpromoting related and the Weiqi is originated from the kidney. Due to the theory we introduce the hypothesis that the essence of the immunity is not in the immunity itself but outside the immunity. To enhance the immunity, we need to pay more attention to the early activation inside the body. Our early study shows that the Warming yang medicine could increase the cellular immunity; and someone also found the target of these medicines was hypothalamus. These findings can verify this hypothesis. Although the Bushen Huiyang Decoction has anti-cancer effect, the mechanism still needs to be verified whether it enhanced the later tumoricidal effect of DC-CIK by increase the proficiency of immunity precursor cells through earlier activation inside the body.The project will imitate tumor patients in immunosuppressed state to establish a mouse model, we will separate the mouse lymphocytes of the late cytokine re-activated DC-CIK cells in vitro amplification and study the Chinese medicine pre-excited DC-CIK could’ improve the ability of multiplication and mmunocompetence in cell level and animal level.We intend to explain the essence of immune regulation outside the immunity itself. Therefore we can find a news break in the discovery of immunity activation.Methods:Used Hydrocortisone prepare immunosuppressive mice model, Compared with the immunosuppressive mice model groups and the blank control group in the appearance, weight and T3 level。The drug were infused to stomach of mice with Chinese Traditional Medicine。Separate bone marrow mononuclear cells and spleen cells to preparat CIK cells。Observe the form, the proliferation and the change of membrane surface antigenResult:(1)Compared with the immunosuppressive mice model groups and the blank control group, the phenomenon ofwet tail was obviously.Their weight had lose 3.19 grams and gain 1.65 grams,the difference was significant.The T3 of the immunosuppressive mice model groups was(0.280±0.032),the blank control group was(0.353±0.047),the difference was significant.(2)DCexpressedCD11c(42.5±1.9)％,CD80(68.6±1.1)％and MHCII(38.3 ±1.3)％.(3)The proliferous multiple of Bushen,Huiyang,immunosuppressive and blank groupwas(1.6±0.3)％、(2.1±0.3)％、(0.4±0.2)％and (3.2±0.2)％。CIK cells of Bushen group,Huiyang group,immunosuppressive group and blank group,the immune phenotype test results were as follows:CD3+cells were(17.03± 1.66)％、(18.02±2.80)％、(10.54±0.76)％、(30.56±1.98)％,CD3’CD8+cellswere(12.58 ±2.00)％、(13.94±2.77)％、(6.31±1.41)％、(17.24±1.39)％, CD3+NK1.1+cells wrere (9.27±1.65)％.(10.71±1.68)％.(4.04±0.74)％.(13.33±0.80)％,the difference was significant.DC-CIK cells ofBushen group,Huiyang group,immunosuppressive group and blank group,the immune phenotype test results were as folloVws:CD3+cells were(17.03±1.66)％、(18.02±2.80)％、(10.54±0.76)％、(30.56±1.98)％,CD3+CD8+ cells were(12.58±2.00)％、(13.94±2.77)％、(6.31±1.41)％、(17.24±1.39)％, CD3+NK1.1+cellswere(9.27±1.65)％.(10.71±1.68)％.(4.04±0.74)％.(13.33±0.80)％. After CIK was cultured with DC cells,The determination of expression the immune phenotype was risen. the difference of Bushen group, Huiyang group, immunosuppressive group and blank group was significantConclusion:(1)Methods Used Hydrocortisone can prepare immunosuppressive mice model successfully.(2)Compared with the immunosuppressive mice model groups and the blank control group,the appearance,weight and T3 level is statistically significant.(3)Bushen Huiyang decoction and Sanyang Xuedai both can activate mouse immune system ahead of time, then boost CIK cell activity.(4)Bushen Huiyang decoction and Sanyang Xuedai both can activate mouse immune system ahead of time,then boost CIK cell activity.(5) Activation ability of Bushen Huiyang decoction is worse than Sanyang Xuedai, the consideration is because the action concentration and magnetism nucleus.