Protective Effects Of Ethanol Extract From Leptospira Interrogans On Chang Liver Cell Injury

Objective:To investigate the protective effect of Rhaponticum uniflorum ethanol extract (RUEE) on oxidative damage induced by hydrogen peroxide (H2O2) in liver cells.Methods:Chang Liver cells were exposed to H2O2 and used as the model of oxidative stress. Then the antagonist effect of RUEE was detected by pretreatment with RUEE (12.5-400 mg/L) prior to H2O2 challenge. Cell viability was evaluated with MTT assay, and levels of LDH, alanine aminotransferase (ALT), aspartate aminotransferase (AST), reduced glutathione (GSH) and malondialdelyde (MDA) were measured by the microplate tecnique. The protein expression of nuclear factor erythroid2-related factor 2 (Nrf2) was determined with immunoblotting method.Results:RUEE had no toxic effect on Chang Liver cells at the concentrations of 12.5～100 mg/L, while H2O2 increased oxidative stress and decreased the cell viability. Compared with the model group, cell viabilities of RUEE group increased in a dose-dependent manner. RUEE also reduced the leakage of LDH, ALT and AST to culture fluid, reduced MDA content, and increased GSH content. The administration with RUEE up-regulated the protein expression of Nrf2.Conclusion:Rhaponticum unifloru has a protective effect on cellular oxidative damage induced by H2O2 in Chang Liver cells.