Fundamental Study On Tissue Engineering Scaffolds Containing Negative Electric Polyethylene Glycol Dioacrylate Hydrogel

Objective: To improve the cell attachment of PEGDA hydrogel, the SMAS was used to modify the PEGDA hydrogel. The charged hydrogel would show improved cell attachment and enhanced protein adsorption which stem from the enhancement of electrostatic adsorption.Methods: In this study, a series of charged hydrogels were produced by adding charged small molecule at different concentrations into the PEGDA solution. We devided the charged hydrogels into four groups, and the unmodified PEGDA hydrogels was chosen as the control group. Then, we investigate the physicochemical and biological characteristics of charged hydrogels, including FTIR, zeta potential, swelling ratio,contact angle, protein adsorption and cell attchment.Results: The FTIR results indicate that the charged monomer had been successfully incorporated into PEGDA hydrogel. And we could effectively regulate electric density with increasing concentration of charge modification. For example, the zeta potential of group HG10(-3.82±0.25 m V) significantly higher than that of HG0. Meanwhile, the protein adsorption of the hydrogel increased with the increasing concentration of charge modification. The amount of protein adsorption of HG10(723.83±33.99mg) increased significantly than that of HG0. The lower water contact angle indicated that the hydrophilia of hydrogel increased. But the water contact angle of HG2.5 changed slightly compared with group HG0. Moreover, compared to PEGDA hydrogel, the cell attchment significantly improved on the charged hydrogel.Conclusions: The charged hydrogel would be a promising scaffold candidate for bone tissue engineering.