Effect Of SMAS Modified PEGDA Hydrogel On The Attachment And Proliferation Of MC3T3-E1 Cells

Objective: To investigate the effect of Sodium Methylallyl Sulfonate(SMAS)modified Poly(ethylene glycol)diacrylate(PEGDA)hydrogel on the attachment and proliferation of osteoblast-like cells(MC3T3-E1).Methods: Negative charged small molecule monomer(SMAS)was added into PEGDA precursor solution.Hydrogels were divided into group HG0?HG50?HG100 and HG200 according to the different concentrations of SMAS.The corresponding final concentration of SMAS was 0m M?50m M?100m M and 200 m M respectively.PEGDA precursor solution was irradiated with 365 nm wavelength ultraviolet rays to make it completely cured in 15-20 minutes.The hydrogel samples were made into the same size using a circular mold which diameter is 2 cm.The hydrogel samples were evacuated,frozen,drying and metal spraying.And then scanning electron microscopy(SEM)was used to observe the surface morphology of the hydrogels.The protein adsorption of the different hydrogels was evaluated by BCA kit.MC3T3-E1 cells in exponential growth period were seeded on the surface of the hydrogels.Cells were observed the growth conditions through the inverted microscope at 2,4 and 8 hours.The cells cultured on the hydrogels were dissociated,collected and counted using cell counter to record the number of cell adhesion at 2,4 and 8 hours.Furthermore,the cell proliferation was investigated by cell counting kit(CCK-8).Results: 1.SEM observations: A large number of uniform distribution 5-10 microns hole structures can be found in group HG0 hydrogels.But 30-50 microns hole structures can be found in SMAS modified hydrogels.2.The determination of protein adsorption: With the continuous improvement of SMAS modified quantity,protein adsorption capacity increased.Compared with group HG0,protein adsorption of group HG100 and HG200 increased 3.7 times and 4.6 times respectively.3.Observation of cell adhesion: Most MC3T3-E1 cells remain spherical on the surface of HG0 hydrogels,but cells preliminary stretched and presented short spindle on the surfaceof HG50,HG100 and HG200 hydrogels at 2h.Only a small amount of cells preliminary stretched on the surface of HG0 hydrogels,but cells further stretched and had short pseudopods extend on the surface of HG50,HG100 and HG200 hydrogels at 4h.All group cells further stretched at 8h.But there were more adhesion cells and better stretch forms on the surface of HG50,HG100 and HG200 hydrogels than HG0 hydrogels.4.Cell adhesion quantity determination: At the three time points,with the increase of the SMAS modification amount,the number of adhesion cells in the early days of the hydrogels surface was obviously more than pure PEGDA hydrogels(p<0.05).The number of adhesion cells on the HG100 and HG200 hydrogels surface was obviously more than group HG0 and HG50.5.The determination of cell proliferation: With the increment of small molecule modification,OD value increased.The group HG50 ? HG100 and HG200 were significantly different compared with the group HG0(p<0.05).At the same time,significant difference had been found between the experimental groups.Conclusion: 1.With the increment of SMAS modification,protein adsorption quantity increased,the number of adhesion cells increased,these all increased the cell proliferation on the surface of hydrogels.3.Negative modification for PEGDA hydrogels modified research provides a new way of thinking.