Direct Electrochemistry Of Hemoglobin Based On Different Modified Electrodes

Hemoglobin is a kind of protein, which has redox activity. Usually, the electroactivity center of Hb is buried in the interior of the protein and there is no direct electron transfer reaction between Hb and electrode surfaces. The immobiliz-ation of Hb on electrode surfaces using appropriate modified matrix is important in investigating the direct electrochemistry of Hb. In this paper, Gemini surfactant, different pore size of SBA-15, Au-[C12min][Br] nanocomposites, ionic liquid and its composite materials are used as the modified materials to immobilize Hb and the reaction mechanism of these materials with Hb are studied. The modified electrodes all show the electrocatalytic effect to H2O2, and can be used to detect the concentration of H2O2.In this paper, Gemini surfactant was used as modified material to promote the direct electronic transfer of Hb. We studied the effect of different surfactant concentrations, different chain lengths and different connection groups of the surfactant to Hb. Five different pore sizes of SBA-15materials were synthesized. The effect of different pore sizes of SBA-15on the direct electron transfer of hemoglobin (Hb) was investigated. We also studied the impact of the ionic liquids with Gemini surfactant and mesoporous materials as compound materials to direct electrochemistry of Hb. Au-[C12min][Br] nanocomposites were synthesized by UV rays and the direct electrochemical behavior of Hb on the nanocomposites modified electrodes was investigated.