The Research Of CAS Gene Transformation Of Tomato

Ginseng (Panax ginseng C. A. Meyer), is five plus branch (Araliaceae), ginseng is Aperennial herbaceous plants, also is herbal medicines valuable. The most inportant activeingredients of ginseng are ginsenosides. Ginseng has a very wide range of pharmacologicaleffects, can fill the five internal organs, combat fatigue, improve memory and improve thecardiovascular system, antitumor and fall hypoglycemic etc.Tomato (Lycopersicon escaleutum. Mill), alias tomato, Solanaceae (Solanaceae) Solanum(Lycopersicon) annual or perennial, is one of the important vegetable crops in the world, and isalso one of the classic model plant, and the main active substance of tomato is lycopene.Tomatoes have hemostatic, blood pressure, stomach and digestion, detoxification, thirstquenching, prevent aging and cervical cancer and other medicinal value.In this experiment, tomato varieties Red Tomato Milk as receptor material, using themethod of Agrobacterium-mediated genetic transformation of tomato research, in order toidentify the ginseng of genome function simultaneously for tomato germplasm innovationThe results of this study are as follows:1. Agrobacterium containing large fragment DNA COR308logarithmic phase for11to17hours, choose13-14hours of agrobacterium experiment.2. Pre-culture time for tomato genetic transformation is2d, infection time is7-8min, totaltraining days for4d.3. The experiments resistant shoots523,32resistant plants by the carrier T zone around thearm PCR, PCR fragment large fragment known CAS detect genes get three positive plants,conversion of0.237%.4. Through the transformed plants and untransformed tomato plants morphological analysis,we find that transformation plants in stem, leaves and sepals form has changed: physiologicallower end of the stem easily transformed plants bifurcation, but not transformed plants do nothave this phenomenon; the stems of transgenic plants to reduce the hair, fluff untransformedplant stems obvious visible; Small leaves of transgenic plants, tomato plants leaves unconvertedbig and wide; sepals transformed plants fruit fluff less transformed plants without fruit sepalsfluff and more.