The Research On The Network Based On Vif-APOBEC3G Axis And Expression Of Related Proteins

APOBEC3G, as an innate anti-HIV-1factor, is selectively packaged into HIV-1virions by interacting with Gag protein. During reverse transcription, APOBEC3Gcatalyzes the cytosine deamination of viral negative strand DNA, thus reducing viralinfectivity. However, HIV-1Vif binds APOBEC3G, with the participation of CBF-β,degrading APOBEC3G through ubiquitin-proteasome pathway and antagonizing itsanti-HIV-1activity. Therefore, HIV-1Vif and CBF-β are the new targets foranti-HIV-1drug research.This study utilized a network visualizing and analyzing software, Cytoscape, tocreate a network based on the Vif-APOBEC3G axis (i.e. APOBEC3G, Vif, CBF-β,CUL5, TCEB2and TCEB1). There were534nodes and679sides in the network.The clustering coefficient was0.165, and the average path length was2.343. Sixputative complexes with the correlation integral value greater than or equal to1were predicted by MCODE plugin-in. Based on previous work in the research group,this study constructed pFC-APOBEC3G, pFC-Vif, pFC-CBF-β and pEGFP-CBF-βeukaryotic expression vector. The images detected by confocal showed that CBF-βfusion protein distributed both in the cytoplasm and nucleus. The expression ofAPOBEC3G was reduced when Vif and APOBEC3G were co-transfected byWestern Blot. There was no detectable APOBEC3G specific band when Vif, CBF-βwere co-transfected with APOBEC3G.