The New Time-resolved Spectroscopy Detection System And Design Research Of The Enhanced Raman Probe

Time-resolved fluorescence immunoassay technology has many advantages,including high sensitivity, strong stability, specificity, and non-radioactive. There isgreat value in medicine, biology and other fields. This paper discusses in detail theanalysis of several immune markers, the excitation and emission spectracharacteristics of rare earth elements and chelates, principles and applications oftime-resolved fluorescence immunoassay technology. We designed and developedtime-resolved fluorescence immunoassay system and achieved the detection of theimmune response on the immunochromatographic test strip. It can determine thesample qualitatively and quantitatively. Experiments show that the minimumdetectable concentration can reach ng/mL of magnitude, and the relative standarddeviation of the measured around2%. It has good reproducibility. Antigen-antibodyreaction of biological experiments has been carried out for effective verification of thewhole system. It achieved non-radioactive super-trace detection.Surface-enhanced Raman Scattering (SERS) can enhance the weak Raman signalobviously, and reduce background fluorescence to some extent. Currently, SERS hastremendous development and becomes more and more popular in biological system ofwater. Suitable enhanced substrate and effective Raman probe can significantlyenhance Raman signal. In this paper, silver nanoparticles were prepared by thechemical reduction method. We proposed a new enhanced Raman probe, whichdetected Rhodamine through the experiment. Experiments show that the new Ramanprobe which we designed has several merits. It is simple, low cost, easy to prepare,widespread use, quickly detected, etc. The minimum detectable concentration ofrhodamine can reach10-9mol/L, which is ten times lower than normal surfaceenhanced Raman probes. So we have made useful exploration in SERS research.