Preparation Of Ginsenoside F1 And Rh1 By Biostransformation

This study focused on the combined enzymatic processes of the major ginsenosides Re and Rg1 for the mass production of minor ginsenosides F1 and Rh1(S). Because ginsenosides F1 and Rh1(S), the pharmaceutical components of ginseng, has been known to have anti-cancer, anti-oxidant, and anti-inflammatory effects, F1 is added in makeup product for whitening and Rh1(S) is one of exceptional component of Korean red ginseng extract. However, the small amount found in ginseng, for getting ginsenoside F1 and Rh1(S), two enzymes which can hydrolyse ginsenoside Re and Rg1 were research from several commercial enzymes. Cellulase KN from Aspergillus niger could effectively transform the ginsenosides Re and Rg1 into F1. The conversion reaction transformed ginsenoside Re to Rg1 using Fungamyl 800 L followed by Rg1 to Rh1(S) using BglBX10 which is conducted by Wan-Taek Im lab.A scaled-up biotransformation reaction was performed in a 10 L jar fermenter to enhance the production of ginsenoside F1 and Rh1(S). The condition of F1 transformation is at pH 5.0 and 50°C for 48 hours with a concentration of 10 mg/m L of protopanaxatriol-type ginsenoside mixture(PPTGM) from ginseng roots. The conversion reaction transformed ginsenoside Re to Rg1 using Fungamyl 800 L in pH 5.0 and 50°C for 48 h, followed by Rg1 to Rh1(S) using BglBX10 in pH 7.0 and 37°C for 12 h.Finally, 13.0 g of F1 was produced from 50 g of PPTGM with 91.5±1.1% chromatographic purity. The yield of F1 is 79.5%. 10.2 g of Rh1(S) was produced from 50 g of Re and Rg1 with 92.6±1.1% chromatographic purity. The yield of Rh1(S) is 62.3%.These results suggest that these enzymatic processes could be usefully exploited in the preparation of ginsenoside F1 and Rh1(S) in the cosmetics, functional food, and pharmaceutical industries.