Research On Microbial Transformation Effect Of Extract From Stem And Leaf Of Notoginsenoside By Bacillus Cereus

According to modern medicine and pharmacology studies, Notoginsenoside has analgesic,hemostatic, lowering blood pressure, prevention and treatment of cardiovascular diseases,anti-inflammatory detoxification effect. This not only makes it attracted widespread attention and efforts to develop, but also stimulates the Notoginsenoside plant cultivation industry. But its development and processing mainly focus on the traditional medicine site-rootscand rhizomes of Notoginsenoside. The Stems and leafs are usually discarded as waste, the annual utilization rate is less than 5% of its production. Recent study found that the active ingredients extracting from stems and leaves of panax notoginseng is stem and leaf saponin which has the similar pharmacological effects with root saponins. The biggest difference with the saponins of Panax taproot is that the chemical composition of stems and leaves of panax notoginseng contains only the protopanaxadiol saponins nearly without panaxatriol saponins. Many monomer saponins can be separated from the stem and leaf saponins. These monomer saponins have excellent anti-tumor biological activity, but these are very little in the stem and leaf, even some rare saponins does not exist. Howerer, with the development of biotechnology, bio-transformation technology has been applied in the preparation of rare ginseng saponin. Rare saponins content can be improved through using the microbial transformation to metabolize the notoginsenoside.In this study, microbial fermentation was applied to the the biotransformation of notoginsenoside. Firstly, we compared four different microbial the transformation effect. Detecteing the total amount of each transformation products by TLC, HPLC method,we found that microorganism Bacillus cereus A8 which was isolated from the soil had the best ability to transform notoginsenoside.Secondly, the study identified the optimal conditions of microbial transforming notoginsenoside:the transforming time 8d, the transition temperature 28℃, the rotating speed 160 r·min-1, the initial pH value 5.5, the substratum concentrations 2% and the strain quantity 15%. Under the optimum transforming conditions, the content of the product T was 40.47 times more than before the transformation. And the transformation rate is 62.25%.At the same time, we also studied the single factor condition of using CH3COOC2H5 to ultrasonically extracting total transformation products. The condition was determined as that ultrasonic time 40 min, ultrasonic temperature50℃, ultrasonic frequency 100kHz, solid-liquid ratiol:10. After extracting by CH3COOC2H5,we got the upper and lower layers samples, numbered T1 and T2.Tumor cell A549,823, MCF-7 and HCT116 were choosen for in vitro activity test of Notoginsenoside,T1,T2,and M. By comparing their in vitro activity,we found that T1 was best to tumor cell HCT116, and IC50 12.36.Therefore, we can say that the transformation ability of strain A8 is strong. The Ag strain has an in-depth research value and development prospects.