Two Year Record (2013 And 2014) Of Archaea Community In Permafrost Soil In Muli Coalfield Of Qilian Mountain, China

As a new emerging potential alternative sources of energy in 21st century, Natural Gas Hydrate has been studied widely. Scientists pay more attention to the gas hydrate occurrence and the relation between gas hydrate reservoir and thickness of permafrost layer in permafrost regions previously. With further research, researchers have realized the importance of permafrost microorganism activity to gas hydrate distribution and permafrost environment change. However, the research still remain rather weak.In this article, soil samples of seven point were obtained in first gas hydrate exploration of permafrost area in China. The microbial abundance and community compositions of these samples were studied by using acridine orange direct count (AODC) and 16S RNA gene phylogenetic analysis.The results shows that archaea in Muli area is mainly composed of Crenarchaeota and Euryarchaeota. The Crenarchaeota contains Group 1.1b、Group 1.3 and a small amount of Thermoproteales. The Euryarchaeota.in this area contains Methanomicrobia、 Thermoplasmata and Methanobacteria. Group 1.3 is the dominant group in whole community. On the whole, the diversity of the surface layer is higher than subsurface,more Group 1.1b was found in surface and more Group 1.3 was found in subsurface generally. Group 1.1b and Thermoproteales has obvious negative correlation with moisture content、electrical conductivity and methane content; Methanogen generally has positive correlation with temperature and methane content. In addition to several points, the distribution characteristics has great inter annual variation in most points, especially the subsurface.Over all, these results provided some data base and reference for the future research of microorganism in permafrost.