The Influence On Selenium,Heavy Metal And Main Bioactive Component Content And Growth Of Selenium Under Different Concentrations Of Gynostemma Pentaphyllum And Ampelopsis Grossedentata

This article belongs to basic application research in which pot experiment is conducted on the special economic plants in Enshi: perennial herbal vine Gynostemma pentaphyllum(Thunb.) Makino and woody vine Ampelopsis grossedentata(Hand-Mazz). Research is made into the change rule of selenium content, content of four kinds of heavy metal like lead、cadmium、mercury and arsenic,main bioactive component content, growth situation of root, stem and leaf in Gynostemma and Ampelopsis under the condition of treatment of different selenium concentrations and specific heavy metal concentration in order to compare the response characteristics of the two plants to the above processing to explore the way of treatment that is fit for its growth and development and can ensure their qualities,and to provides the basis for quality control as well as the development and utilization in the process of large-scale selenium cultivation of Gynostemma and Ampelopsis.The main results are as follows:1. With the increase of the concentration of selenium treated, the selenium contents of the two kinds of plants are in curve changes. The selenium content of Gynostemma has the rule of first rise then fall. When the concentration of selenium treated reaches26 mg/kg, the selenium content reaches the highest level, significantly higher than that of the control group. The selenium content of Ampelopsis has the rule of first fall then rise. When the concentration of selenium treated is 2 mg/kg, its selenium content is the lowest; When the concentration of selenium treated is higher than 30 mg/kg level, its selenium content is significantly higher than that of the control group.2. The processing of different selenium concentrations has different effect on the absorption of Gynostemma and Ampelopsis of heavy metals lead, cadmium, mercury,arsenic. Processing of a certain selenium concentrations plays significant inhibitory role in Gynostemma’s absorption of heavy metals lead, cadmium, mercury, arsenic.The selenium concentrations in the range of 6-10 mg/kg and 36-70 mg/kg reflect inhibitory effect on lead absorption. The selenium concentrations respectively in the range of 6-70 mg/kg, 36-70 mg/kg and 6-70 mg/kg reflect inhibitory effect on the uptake of cadmium, mercury, arsenic.The processing of different selenium concentrations has significant inhibitory effect on Ampelopsis ’ absorption of mercury, while it plays significant promoting role in Ampelopsis ’ absorption of heavy metals lead, cadmium, arsenic. The selenium concentrations in the range of10-50 mg/kg reflects the promoting effect of Ampelopsis on absorption of lead. The selenium concentrations in the range of 0.5-50 mg/kg reflects the promoting effect of Ampelopsis on the absorption of arsenic and cadmium, while The selenium concentrations in the range of 1-50 mg/kg reflect inhibitory effect of Ampelopsis on mercury.3. Treatment of selenium with a certain concentration can obviously promote the accumulation of saponins in Gynostemma and flavonoids in Ampelopsis. The influence of increase of Selenium concentrations on the total saponins content in Gynostemma blade takes on the rule of first inhibition, then promotion, and at lastinhibition. Selenium with Concentration of 6 to 20 mg/kg and more than 40 mg/kg plays significant inhibitory role in the total saponins content in Gynostemma blade.Selenium with Concentration 26 to 30 mg/kg of has significant role in promoting the total saponins content in Gynostemma blade. When Selenium concentrations is 26mg/kg,the content of total saponins is the highest 86.46 mg/g.Selenium mainly inhibit the increase the total saponins content in Ampelopsis. Except that Selenium with concentration of 30 mg/kg can significantly promote the total saponins content in Ampelopsis,(the flavonoids content is highest, 104.46 mg/g), selenium with other concentrations has inhibiting effect on the total saponins content in Ampelopsis.4. Treatment of selenium with a certain concentration has a significant role in promoting the growth of root, stem and leaf of Gynostemma and Ampelopsis, and has significant influence on the flowering, inflorescence and weight. Selenium with Concentration of 2 mg/kg significantly improve the increase of Gynostemma in root length, total surface area, root and lateral root number. Selenium with Concentration of 2-50 mg/kg and 26-40 mg/kg significantly improve respectively the increase of Gynostemma in Stem elongation and biomass. Selenium with Concentration of 2-50mg/kg and 6 mg/kg promotes the increase of Gynostemma in leaf number and leaf area on average. Selenium with concentration of 26 mg/kg promotes the increase of Gynostemma in leaf biomass. Selenium with Concentration from 1 to 20 mg/kg significantly promote the increase of Ampelopsis in root length, root surface area, root volume, root and lateral root number. Selenium with Concentration of 0.5 to 5mg/kg with 10 mg/kg respectively promote increase of Ampelopsis in stem elongation and biomass. Selenium with Concentration of 0.5 to 10 mg/kg and 10 mg/kg respectively promote the increase of Ampelopsis in leaf number and growth of average leaf area. Except that Selenium with concentration of 10 mg/kg has no significant effect on biomass of Ampelopsis, Selenium with other concentration all inhibits the increase of biomass.In addition, treatment of selenium with different concentrations can have the effect on delaying florescence of Ampelopsis. Selenium with concentration of 1-20 mg/kg significantly promote the increase of Ampelopsis in inflorescence and weight.