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The Study Of Antioxidant Enzyme Genes Change In MET Period Of Mouse Embryos

Posted on:2017-05-02Degree:MasterType:Thesis
Country:ChinaCandidate:Y Y JingFull Text:PDF
GTID:2180330488456828Subject:Animal breeding and genetics and breeding
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In this experiment, early embryos in mouse as experimental material, detecting the the level of ROS and the expression quantity of antioxidant enzyme gene and peroxisomal membrane protein-20(PMP-20), therefore investigated changes of peroxisome in amount in MET period of mouse early embryos. Moreover, detected the effect of active oxygen (for example H2O2) on the changes of the expression quantity of antioxidant enzyme gene and peroxisome in this period. This study can lay the foundation for the antioxidant mechanism of early embryos in mammalian. Results are as follows:1、Respectively treated the mouse embryos with the concentration of 0, 10-5%,10-7%,10-9%of H2O2, determine that the effective concentration of H2O, of mouse embryos in vitro development was 10-7%.2、In the vivo culture group, the active oxygen content within 4-cell embryo was significantly higher than that within 2-cell early and late embryos, and the active oxygen content within 2-cell early and late embryos was no significant difference; In both vitro culture group and H2O2treatment group, all were that the active oxygen content within 2-cell late embryo was significantly higher than that within 2-cell early and 4-cell embryos, and the active oxygen content within 2-cell and 4-cell embryos was no significant difference. In the same period of 2-cell early and late embryos, the level of active oxygen content in vitro culture group was significantly higher than that in vivo culture groups, but the level of active oxygen content in vitro and vivo culture group was no significant difference3^ Whether early embryos were treated with H2O2 or not, the expressions of CAT, GPX, Mn-SOD, GPX-1、GPX-4 and PMP-20 genes in 4-cell embryo were higher than 2-cell late and early embryos. But the CAT gene did not express within 2-cell embryo, the expressions of Mn-SOD, GPX-1, PMP-20 genes in 2-cell early embryos all were higher than that in 2-cell late embryos, the expression of GPX-4 genes in 2-cell early or late embryos was no difference; And the expression of Cu/Zn-SOD gene within 4-cell embryo was significantly lower than 2-cell early and late embryos. But in the vitro culture treatment group, the expression of Cu/Zn-SOD gene in the 2-cell early embryos was significantly higher than that in 2-cell late embryos, and H202 treatment group was on the other hand;4、In the same period, the expressions of Mn-SOD, GPX-1 and PMP-20 genes in the H202 treatment group were higher than that in vitro treatment group; only in the 4-cell period, the expressions of CAT, GPX-4 genes in the H202 treatment group were higher than that in vitro treatment group; the expression of Cu/Zn-SOD gene in vitro treatment group was significantly higher than that in the H202 treatment group only in the 2-cell early embryos.Conclusion:The expressions of different antioxidant enzyme genes were different in MET period. The expression of PMP-20 gene was trough in 2-cell late embryos, indirectly showed that the amount of peroxidase was deficiency in this period.
Keywords/Search Tags:Mouse, Early embryo, Antioxidant, Antioxidant Enzyme Gene, Peroxisome, MET
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