Design And Implement For Fully Automatic Nucleic Acid Extraction System Based On Magnetic Separation

Nowadays, nucleic acid testing (NAT) has been playing an increasingly important role in the field of life science and clinical assay owing to its high sensitivity, excellent specificity, no window phase and other significant advantages. With the continuous development of NAT, researchers expect to complete the detection research of vast samples in a short time. While the nucleic acid extraction technology, the primary key of NAT, has become the bottleneck to restrict the NAT development and further extending application. The development of nucleic acid extraction technology is directed towards the higher efficiency, higher speed and automation. Therefore, it is extremely necessary and urgent to investigate and implement automatic nucleic acid extraction system.Based on the major demands listed above and under the design principle of "methods were researched firstly, and then the corresponding instrument was developed secondly", this thesis puts forward a complete set of automatic nucleic acid extraction system design scheme based on magnetic separation and analysis research and design deeply surrounding the principle and technology of core method, function module, mechanical structure and control system of automatic nucleic acid extraction system. With the 96-well plate as reaction vessel for its universality in biochemical experiment, targeted magnet bar type magnetic separator is designed. This thesis introduced several technologies, including motion control technology, temperature control technology and man-machine interaction technology, and these technologies are efficiently combined to develop the fast and efficient automatic nucleic acids extraction system with independent intellectual property rights and own patented techniques, where nucleic acids can be simultaneously ectracted from 16 samples and meet the requirement for field-portable rapid sample processing. In order to solve the current bottleneck problem of automatic nucleic acid extraction, embedded control platform based on μCOS-Ⅱ real-time operating system is constructed which achieves high efficient driver of three function modules and real-time monitoring alarm for system with completing the hardware circuit design including the power module, motor drive, sensor acquisition, communication module, temperature control and software programming.After the completion of manufacturing and assembly for each function module of the automatic nucleic acid extraction system, the hardware and software debugging for the control system, the whole blood DNA extraction experiment is conducted to validate the overall performance of the system according to the design target. The results show a promising application prospect with high degree of automation, stable result and simple operation.