Effect Of Phenol And Hydrargrum Ion On The Antioxide Enzymes And Gene Expression Of Metallothionein In Eisenia Foetida Earthworm

The aim of this study was to explore the acute toxic effects of phenol and hydrargrum pollution on earthworm through detecting the changes of some important antioxide enzymes and metallothionein(MT)gene expression in Eisenia foetida. The filter paper contacting test had been used to detect the physiological growth conditions, fecundity and the activity of superoxide dismutase(SOD), peroxidase(POD)and catalase(CAT)in E.foetida which had been exposing to 0.1 g/L, 0.2 g/L, 0.3 g/L, 0.4 g/L, 0.5 g/L phenol and 0.02 g/L, 0.04 g/L, 0.06 g/L, 0.08 g/L, 0.10 g/L mercuric chloride for 24 h, 48 h and 72 h respectively. Meanwhile, the expression of MT mRNA had been determinated by RT-PCR in E.foetida which had been exposed to phenol and mercuric chloride for 48 h.All the datas had been done the prominence analysis on the level P<0.05.The results suggested that there were obvious injured effects of high concentration phenol and mercuric chloride on the physiological growth conditions and fecundity in E. foetida, and the stress time was longer, the damage was more serious and irreversible. When E. foetida had been exposed to phenol, the SOD activity was prominently activated at low concentration and inhibited at high concentration in short time and inhibited in long time, and the SOD2 activity was markedly inhibited in SOD isozyme; the POD activity was significantly activated at low concentration and inhibited at high concentration, and the main change of POD isozyme was that the POD1 activity was significantly inhibited; the CAT activity was activated evidently, but the CAT isozyme had no obvious changes; the expression of MT mRNA was induced prominently(except 0.2 g/L). However, when different concentration of mercuric chloride was used to stress E.foetida, the SOD activity was significantly activated at low concentration and inhibited at high concentration, and even the SOD2 activity was completely inhibited at high concentration in longer period; the POD activity was the same as SOD, some new POD isozymes could be induced and the kind of new isozyme was different in different period; the CAT activity was prominently inhibited at 0.04～0.06 g/L and activated at all the else groups, but the CAT isozyme had no obvious changes; the MT mRNA expression was significantly induced.