Preparation And Characterization For Porous Homogeneous-composite Membrane Reinforced With Wool Keratin-whisker

By using wasted hair fiber as a recycling resource to prepare keratin compositemembranes, i.e. the fibrils compound into the substrate obtained by the way of part separationand complete dissolution of the fiber, respectively, and then through the ways of porous,homogeneous blending, or mixed composite with keratin fibrils (whiskers) for this kind ofrecycling materials, and try to prepare medical-used materials and the study on its formability.Through the single factor test and orthogonal test, and forming preparation andmeasurement for the keratin membrane, several important experimental results have beenobtained.1. Choose strong polar neutral solution to prepare wool keratin solution. According to thestandard curve of keratin concentration, we can calculate the concentration of the keratinsolution after dialysis is between 3-5mg/ml, and it can be reach 50mg/ml after concentration.Also, the content of SDBS calculated in the original solution is little enough within thedialysis time.2. Choose physical-chemistry method to remove wool scale and extract the cortical cellsof wool fiber. The results show that the optimal parameters of removing wool scale are formicacid pretreatment time 15min, ultrasonic working time 27min, power of ultrasonic 200W,ultrasonic working time (3s) / stopping time (6s); the optimal parameters of extracting corticalcells are ultrasonic working time 60min, power of ultrasonic 600W, ultrasonic working time(3s) / stopping time (6s);3. Choose lyophilization method to prepare wool keratin porous membrane andkeratin-whisker homogeneous compound membrane. According to the SEM pictures, it can beknown that the values both of liquid concentration and frozen temperature are inverselyproportional to those of porous membrane aperture and porosity, but proportional to theporous membrane density. Frozen temperature has little impact on the porosity because thesame concentration liquids. The pore of the membrane has a dual character: the front side hasbetter compactness than the reverse side.4. The result of FITR shows that porous membrane, compound membrane and wool arenearly the same. In the compound membrane, whisker and macromolecules are physicalcombined with no chemical bond changes. DTG analysis indicates that the maximum distinctdifferential peak of porous membrane at 327℃; the first distinct differential peak of thecompound membrane is range of 30~100℃, the second is 230~545℃,and the maximum oneis 321℃and 502℃,respectively. The first peak approaches to that of the porous membrane. It is evident that the first DTG peak indicates the thermo-degradation of keratin matrix and thesecond one stands for the thermo-degradation of keratin fibrils, that is the compoundmembrane is deffirent from the porous membrane even the same keratin fiber.5. Comparing the mechanical property between porous membrane and compoundmembrane, it shows clearly that cortical cells can enhance the mechanical property of porousmembrane, and the higher the content of cortical cells, the better mechanical property ofkeratin membrane will be.