Determination Of Some Sulfonamides, Fluoroquinolones And Cephalosporins Drugs By Capillary Electrophoresis

Abuse of many antibiotics has a serious threat to people’s physical health, experts pointout that, often eat the food with antibiotics, even trace, also can make a person appearurticaria or cause allergic shock, and still can make certain strains of bacteria resistant to bringforth the difficulties of prevention and treatment of some people and livestock disease. Thecountries all over the world to use of antibiotics put forward strict standards, the EuropeanUnion’s standard is more strict than the Japanese and Americans, the limited value is0.1mg/kg. We have to strengthen our own testing ability in order to ensure our food safety andexport products to the above country,This paper not only study drugs in body fluids of infected people by capillary zoneelectrophoresis method, but also establish the method to determine antibiotic residues inenvironmental samples and aquatic products with a new type of sample preparation methods-accelerated solvent extraction(ASE).Chapter one: The capillary electrophoresis technique, accelerated solvent extraction andapplication were described simply. The analytical research and development status ofcephalosporin, sulfonamides and fluoroquinolones were reviewed.Chapter two: A novel capillary zone electrophoresis (CZE) method is, for the first time,developed for effective separation and simultaneous determination of ceftriaxone sodium andlevofloxacin in human urine. The electrophoresis conditions were investigated and optimized.Good linearity of quantitative analysis was achieved with the correlation coefficients (r)of>0.999. The limits of detection (LOD) for ceftriaxone sodium and levofloxacin were2.5and7.5μg mL1, respectively. Their average recovery from human urine was99.15%withthe RSD of0.651.54%for ceftriaxone sodium, and99.57%with the RSD of0.22.0%forlevofloxacin. The proposed method is simple, rapid and accurate, and provides the sensitivityand linearity necessary for selective and simultaneous analysis of the test drugs in humanurine at clinically relevant concentrations. Chapter three: A new accelerated solvent extraction (ASE) capillary electrophoreticmethod was developed for determination of sulfonamide residues in soil and bottom sediment.The method provides a rapid and simple extraction procedure with high recoveries, and hasbeen applied for sample pretreatment and determination of sulfonamide residues in soil andbottom sediment.Chapter four: A rapid and effective accelerated solvent extraction (ASE) capillaryelectrophoretic method was developed for the determination of fluoroquinolone andsulfonamide residues in aquatic products. The electrophoresis and extraction conditions wereinvestigated and optimized. The method provides a rapid and simple extraction procedurewith high recoveries, and has been applied for the determination of4fluoroquinolone and3sulfonamide residues in aquatic products with satisfactory results.