Study On Microbial Preparation Reduce Affect To Nutrients Of Gulf Sediments

Nitrogen and phosphorus are the primary nutrients sea water. In practice,preservation of nutrients has been a key factor affecting water quality for the study ofhigh salinity of nutrients preservation techniques.In this paper,12different preservationmethods was used to save4kinds nitrogen and phosphorus nutrients in salinity of32high salinity sea water. We studied the9days storage parameter values’ stability of fourkinds nutrients (NH4+-N, NO2--N, NO3--N, PO43--P) using FIAstar5000nutrient flowinjection analyzer. The results showed that there were obvious differences betweendifferent preservation methods, the best preservation conditions of each nutrient wasdifferent. In9days experimental period, NH4+-N in water samples had a large changetend, the NH4+-N and PO43--Pcontent presented a trend of decrease, while NO2--N andNO3--N content had slightly rose. When added chloroform into water samples it wasvery good for the preservation of nitrogen nutrient (NH4+-N, NO2--N and NO3--N),when added formaldehyde it was good for the preservation of phosphorus nutrient(PO43--P). Considered the effectiveness and operability of the experiment results, weobtained the optimal preservation condition of4diffident kind nutrient in9days: whenadded5‰chloroform into water samples the stability of NH3-N nutrient could becontrolled more than80%; when added1‰chloroform into water samples and storedthe water samples at-20℃,the stability of NO3--N nutrient could over95%; when added5‰chloroform at-20℃or added1‰chloroform at4℃into water samples theNO2--N nutrient could be stabled at more than95%; when added5‰formaldehyde at4℃it was the best method of save PO43--P nutrient.We assumption the internal migration of the gap between sediment and nutrients inthe water to Dapengao of Daya Bay as the study area.The bay sediments and overlying water collected on the original, in the laboratory to simulate natural state, timingacquisition overlying layer of water and5collecting interstitial water measurement andanalysis NH4+-N、PO43--P、NO3--N nutritious salt content changes. The results showedthat three kinds of overlying water nutrient concentrations are far worse than Class IVwater quality standards, interstitial water content of each nutrient was significantlyhigher than the overlying water, and the content increases with increasing depth;overlying water three kinds of nutrient content both showed increasing trend over time.2cm above the sediment NH4+-N content decreases with time,2cm or less NH4+-Ncontent increased with time; sediment pore water NO3--N and PO43--P concentrationsare increasing. a layer, b layer overlying water and sediment pore water NH4+-N,NO3--N content showed a significant correlation, c layer, d pore water layer overlyingwater and sediment pore water NH4+-N, NO3--N levels were not significantly correlated,the overlying water PO43--P content and a, b, d layer interstitial water PO43--P content ofthe correlation is not significant, only c layer overlying water and pore water has asignificant linear relationship (R2=0.4822, P <0.01), c and d layer interstitial waterlayer NH4+-N and PO43--P content has a significant linear correlation coefficient was0.885(P <0.01) and0.836(P <0.01).The results showed that factors affect nutrientchanges-in overlying water are various，in the condition of high concentration gradient,sediment are the source of NH4+-N、PO43--P and NO3--N in overlying waterIn order to study the change of nutrients between interstital water and overlyingwater in bay sediments under the agents of microbial. Under the condition of thelaboratory simulation of natural sea waters, Join the Microbial preparation in theoverlying water of gulf argillaceous sediments and sandy sediments respectively. Byperiodically measuring the experimental and control groups four nutritious (NO2--N、NO3--N、NH4+-N、PO43--P) content with time series variation in stratified sedimentinterstitial water and the overlying water to study the impact of microbial preparationfor the nutrients changes in water-surface sediments interface. Experimentaldetermination of9days showed that the NH4+-N content in overlying water ofexperimental group in an overall reduction, including mud, sand in the experimentalgroup on the overlying water NH4+-N content decreased by1.686mg.L-1and1.099mg.L-1, The NO3--N content in overlying water of argillaceous and sandy sediment were increased by0.070mg.L-1and0.085mg.L-1, and the PO43--P content were elevatedamount of0.070mg.L-1and0.085mg.L-1. Indicate microbial agents to promote thedegradation of organic matter in surface sediments and sandy sediments averagedegradation indicators better than argillaceous sediments. To end of the experiment thesediment surface to form a layer of grayish yellow0.5cm oxide layer. Studies haveshown that microbial preparation can degrade the water and sediments of ammonianitrogen and organic phosphorus.