Preparation Of Sodium Copper Chlorophyllin And Extraction Of Other Bioactive Compounds From Microalgae

Microalgae, capable of performing photosynthesis, are some of the fastestgrowing organisms which consist of chlorophyll a in the world. Microalgae can beproduced using a wide variety of methods, ranging from closely-controlled laboratorymethods to less predictable methods in outdoor tanks. Microalgae are made up fromcarbohydrate, protein and oil. As well as microalgae are also rich in high-valuecompounds, such as pigments and biologically active compounds. Thecomprehensive utilization of microalgae has important practical significance toreduce production cost and fully utilizing resources.In this thesis the traditional preparation process of sodium copper chlorophyllsalt was improved. The new method is benefit to protect extracted chlorophylldamage, increase the yield and the quality of the products, and get more stable copperchlorophyll acid. To optimize the preparation process of sodium copper chlorophyllsalt, single factor experiments were be done. The results of experiments show that:under100g microalgae raw material, ultrasonic processing30min,85℃extract5h,copper generation for the first time in25ml5%CuSO4solution, saponification timefor60min, saponification temperature is70℃, with0.5mol/L KOH to adjust thesaponification C2H5OH solution pH in11, then5ml10%CuSO4solution addedagain, the yield was85.3%. The quality of sodium copper chlorophyll salt productionreaches the national standard GB3262-1982.Using the method of microwave the pectin was extracted from algal residue. Theresults of experiments show that under500ml of30%dilute HCl,720W microwaveextraction10min, activated carbon45g/diatomite5g adsorption decolourization0.5h, the ratio of raw material to95%ethanol=1:3,30g raw material produced3.97gpectin. The yield was13.83%. The pectin products are up to standard GBn246-85HMP.Beta-carotene and lutein were separated by thin-layer chromatograph. The eluentwas petroleum ether-acetone (9:1).