Study On The Determination Of UV Filters In Hygienic Standard For Cosmetics

The main components of the sunscreen products are the UV absorbers. There are sometypes of the UV absorbers, such as chemical synthesized and the natural extraction UVabsorbers. The chemical synthesized UV absorber are added in the cosmetics in themarket.But there are certain dangers if human excess absorberd.Fifteen UV absorbers havebeen limitted contents in the “Hygienic Standard for Cosmetics”(2007edition), and the testmethod are also setted. Dection methods of the fifteen UV absorbers are also reported in theliteratures, and detection methods other than fifteen UV absorbers rarely reported.The“Hygienic Standard for Cosmitics”(2007edition) only include a few types of UV absorbersand has no clear explanation treatment of the different forms of cosmetics.Therefore there arelots of disadvantages of the standards and it can not meet the demand in the daily detection.This paper is selected four major types of chemical synthesis of13sunscreen UV absorberswhich not included in the “Hygienic Standard for Cosmetics”(2007version) and establishpre-treatment metnods for different different types of sunscreen products. The results are asfollows:In this study, a high performanece liquid chromatography method was developed andevaluated for determination thirteen UV absorbers. Samples pretreatment were processed asfollows:1. Liquid, oily sunscreen products: After a certain amount of samples extracted withmethanol, Adding over amount anhydrous sodium sulfate in order to centrifugation the water.Take the supernatang liquid for the chromoatography to qualitative and quantitative.2. Cream sunscreen products installed: After a certain amount of samples extracted withmethanol, after Si solid-phase extraction (SPE), n-hexane-dichloromethane (1:1, V/V)eluting rinsed, dried by nitrogen enrichment,separation the target compound with themethanol carried by HPLC column separation and UV detection. Take the supernatang liquidfor the chromoatography to qualitative and quantitative.In addition to separate and determind the target compounds are as follows:Column:DIKMA DIAMONSIL C18column; column temperature:25℃; mobile phase: methanol-1%methanoic acid water; gradient elution: from0.00min to3.00min at40%methanol. From3.10min to5.00min methanol sharply change to80%and until to10.00min. From10.01minto12.00the methanol changed to95%and until to20.00min.Finally from20.01min to22.00min the methanol changed to40%and until to25.00min; We set the ultraviolet wavelength at311nm at the beginning, and swithed to254nm from8.00to10.00, and finally311min until30.00min.13kinds of correlation coefficients range of UV absorbers standardcurve ranged from0.9910to0.9995;The relative standard deviation (RSD) ranged of themethod ranged from1.75%to7.63%; The detection limit of the method ranged from0.5mg/Lto20mg/L; the recoveries ranged from84.96%to103.68%.35cosmetics samples were successfully determind by the established method.Themethod advantages are the treatment is very simple, the test result is reproducible andaccurately, cost is low, small impurity interference characteristics, suitable for batchdetermination for sunscreen products in a variety of UV absorbers.