Research On Detection Method Of Cephalosporin Titer In Biopharmaceutical Residue And Resource Feasibility Study On Residue

Cephalosporin slag is a kind of solid waste, which generated in the production of cephalosporin C fermentation process, because it contains trace amounts of cephalosporin C residues, existing potential hazards on the ecological environment has been designated as hazardous waste. Current research focus is in the field of testing and rational utilization of residual slag technical bacteria titer. Based on the physicochemical properties of cephalosporin slag analyzed to how to detect residual slag cephalosporin titer and bacterial residue utilization for research purposes, by bacteria slag composition analysis, the use of cephalosporin in a large number of lipid residue, proteins and other organic substances, waste of resources into the purpose of this paper, an independent study of the cephalosporin residual slag detection methods and full potency cephalosporin anaerobic fermentation residue pretreatment process for subsequent research and resources to solve the bacteria residue provide the basis for utilization has great reference value.First, the physical and chemical nature of the residue analysis cephalosporin, cephalosporin slag mainly inorganic elements C, H, O, N, S, cephalosporins accounted for94.4%of the dry weight of slag; bacteria residue Fourier transform infrared analysis showed cephalosporin slag mainly containing CN, C=C,-SS-groups, such as alkanes, lipids, proteins and other organic matter; cephalosporin dry residue of crude protein content of39.37%,16.55%crude fat, moisture content of62%; Finally, the cephalosporin slag content analysis of some metal elements, including Ca, Mg high element content, Cu, Cr, Cd, Pb, Ni and other heavy metal content is very low, lower than GB18918-2002as specified in the sludge at pH<6.5acidic soil contamination control standards when used in agricultural value.Secondly, the detection methods establishment of residual titer slag cephalosporin cephalosporin C, the establishment of a high-performance liquid chromatography method cephalosporin C standard, the choice of Agilent C18(250mm×4.6μm) column, acetonitrile:10mmol/L NaH2CO3(0.2:0.8) as the mobile phase flow rate of1.0mL/min, column temperature was30℃, UV detector, detection wavelength254nm, standard curves using the above condition is detected cephalosporin C with high accuracy, R2=0.9995; extraction method to determine the residual slag cephem cephalosporin C, a mixture of40%aqueous acetonitrile as the extracting agent, ultrasonic extraction, and extracted three times, each time15min, extract pH value of6, n-hexane extraction and purification rotary evaporation, extraction rate guarantee between80%to120%, and finally, the detection methods were established to verify the actual sample, indicating that this method is better for adaptive real samples, the minimum quantitative detection limit was10.8μg/kg, qualitative detection limit was3.2μg/kg, were far below the maximum residue limit value cephalosporin antibiotics in animal food EU regulations.Finally, in order to improve cephalosporin slag the biodegradability, respectively cephalosporin joint three pretreatment hot slag, alkali, alkaline hot slag after pretreatment of bacteria producing methane biochemical experiments to determine the amount of alkali0.08gNaOH/gTS, treatment temperature80℃, the combined treatment two hours, as the best pretreatment and methane yield of450mlCH4/gVS,1.34times the yield of methane cephalosporin slag untreated. cephalosporin C were not detected in anaerobic fermentation of the remaining residue, biogas slurry.