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Study On Extraction And Characterization Of Black Rice Anthocyanin

Posted on:2015-07-27Degree:MasterType:Thesis
Country:ChinaCandidate:X L BaoFull Text:PDF
GTID:2181330422976588Subject:Food, grease and vegetable protein engineering
Abstract/Summary:PDF Full Text Request
With black rice as raw material, the use of microwave-assisted process tooptimize the optimum the best extraction technology and ultrasonic synergiescompared to black rice anthocyanin extraction, microwave extraction of antioxidantresearch and study the performance and stability of anthocyanins technology,beneficial plant. The main research results of this paper is as follows:1.Through the same test conditions for ethanol, acetone, ethyl acetate, and byusing60%ethanol, acetone, ethyl acetate with anthocyanin content of average of0.3,0.73,0.26, respectively. So,choose acetone as extraction solvent..Through theresponse surface analysis method of microwave assisted technology is optimized, theresults show that the volume fraction is88.88%acetone92.84s, microwave power,microwave time is471.13W, extracting anthocyanins is1.5,Black rice wasoptimized by using the response surface method in the optimum process conditionsof anthocyanins, significantly improve the extraction yield, under the conditon ofoptimum process, the ultrasonic time is54min, acetone volume fraction is82%, themicrowave power is480w, and measured black rice anthocyanins extraction yield of2.411%.2.This paper adopted the scavenging free radicals in vitro determination ofblack rice anthocyanins oxidation resistance test, anthocyanins total antioxidantactivity, remove ability on superoxide anion and cleaning DPPH free radicals and theability to do the comparison, the results found that under the same experimentalconditions, the total resistance to oxidation and removal of superoxide anion freeradical ability is stronger than vitamin C, but the ability of DPPH free radical thanvitamin C ability is poor.3.Respectively using different storage conditions of illumination, pH,temperature, metal ion to do the research, among them, avoid light, pH is acidity,temperature, when the deposit is lower anthocyanin loss rate is low, and the presenceof metal ions, Cu2+, Fe3+, Sn2+exist, more obvious influence on stability ofanthocyanins.
Keywords/Search Tags:Microwave extraction, ultrasonic extraction, oxidation resistance, stability
PDF Full Text Request
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