Modification Of Lactobacillus Peptidoglycan And Its Effect On The Study Of Mouse Intestinal Mucosa Protection

Peptidoglycan is an important part of the bacterial cell wall. In this thesis, Lactobacillusacidophilus cell wall peptidoglycan as the object of study. Peptidoglycan of lactobacillusacidophilus cell wall was extracted, separated and purificated in this study. At first, cellwall was broken, and then the teichoic acid, protein and fat were removed in order. Finally,the dialysis freeze-dried to obtain purified peptidoglycan. The TEMPO/NaClO/NaBrcatalytic oxidation system of the purified peptidoglycan was modified to improve thesolubility of peptidoglycan; Then the content of soluble sugar modified peptidoglycananalysis,modified solble peptidoglycan have greatly improved.Of lactobacillus acidophilus cell wall peptidoglycan modified the best processconditions were studied. By single factor experiment, determine the reaction temperature,reaction time, concentration of substrate scope, then best oxidative modification conditionswere determined by orthogonal experiment. The results show that the reaction temperature,reaction pH, NaClO content significantly affects the degree of lactobacillus extracellularpolysaccharide selenide, when the reaction temperature5℃, pH8.5add quantity is2.5mg/ml, NaClO soluble sugar content reaches the maximum value.After studied the selective oxidation of peptidoglycan in vivo and in vitro antioxidantactivity. Through animal experiments, detection in the serum and liver tissue of micehydrogen peroxide enzyme (catalase, CAT) energy, gsh-px activity, T-AOC vitalitymalondialdehyde, malondialdehyde (MDA) content, superoxide dismutase (SOD), superoxide dismutase, SOD) the results showed that the gastric selective oxidation ofpeptidoglycan mice compared with the control mice, significantly higher antioxidantcapacity. To study the selective oxidation of peptidoglycan of of the protective immune injury caused by cyclophosphamide, the results showed that extracellular polysaccharideselenide can significantly antagonism immune injury in mice spleen index and thymusindex, liver index fell, and to combat the decline of serum hemolysin caused bycyclophosphamide, therefore, oxidation peptidoglycan have protective effect on immunefunction injury caused by cyclophosphamide.Peptidoglycan in mice infected with pathogenic escherichia coli were studied intestinalmucosa secretory immunoglobulin (SIgA), serum interferon-IFN-γ(IFN-IFN-γ) andtumor necrosis factor alpha (TNF-α) and intestinal mucosal thickness and villus heightand crypt depth, explore the peptidoglycan of pathogenic escherichia coli infection miceintestinal mucosal protective effect and mechanism.