| Exopolysaccharides (EPS) can be vastly produced by lactic acid bacteria during its growth. Thefermentation broth of Lactococcus lactis subsp. Lactis was collected to isolate exopolysaccharides andeffective selenium (Se) dietary supplement was produced.It is well known that lymphocytes play an important role in the immune response, and that theactivation of lymphocytes is an important event after infection in relation to the proliferation and release ofcytokines. The activities in lymphocytes were related to intracellular complex signal channels. Calciumsignaling channels play a pivotal role in the activation and proliferation of lymphocytes. Even minutechanges in the level of intracellular Ca2+can lead to cellular activity in the calcium signaling channel. Insome tumour cells, the concentration of Ca2+can reveal the cells condition. Some moleculars, for instancecyclic AMP (cAMP) and cyclic GMP (cGMP), were crucial to the cellular signal transduction system.Studies of the change in cells stimulated by se-exopolysaccharides would contribute to its mechanismresearch. Analysis of the Se content and monosaccharide components of Se-EPS demonstrated that itconsisted of mannose, fucose, ribose, glucose, galactose, and arabinose with a molar ratio of5.48:0.39:9.77:4.03:1.00:1.92. Se-EPS could affect the immune function of mouse spleen lymphocyte by release ofcytokines, which may be one of the mechanisms.The effect of Se-EPS on intracellular Ca2+level in mouse macrophages, gastric cancer cell SGC7901and Hela cell were studied by marking the cells with the Fluo-3/AM dye and examined using laser confocalscanning microscope. The intracellular Ca2+level in mouse macrophages increased gradually. Se-EPSdecreased the intracellular Ca2+level both in gastric cancer cell SGC7901and Hela cell. Se-EPS activatedmacrophage may relate to Ca2+pathway. We hypothesized that through Se-rich modification the EPSgained the capacity of apoptosis of tumor cell. |
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