Applications Of Tris(2,2’-bipyridyl)Ruthenium(Ⅱ) Chemiluminescence In Pharmaceuticals Analysis

In recent years, Ru(bpy)32+have been widely used in chemistry, biology, material,electronic and other fields due to its advantage in optical and electrochemical aspects.While electrochemiluminescence (ECL) is an effective trace analytical technology becauseof the integration of high sensitivity and controllability of potential. Combining of them,Ru(bpy)32+electrochemical analysis technology will have broad application prospects. Inthis dissertation, Ru(bpy)32+act as ECL reagent. We construct different Ru(bpy)32+ECLmethods for the detection of etamsylate, atropine, tramadol and lidocaine, which are usedas preoperative medication, respectively.The dissertation includes three chapters:In chapter1, the significance and background knowledge of this dissertation havebeen introduced with the emphasis on the following aspects, which is the properties ofRu(bpy)32+, fundamental principle of Ru(bpy)32+ECL system and the application ofRu(bpy)32+ECL in the pharmaceutical analysis.In chapter2, a electrochemiluminescence (ECL) method for the determination ofetamsylate has been developed by coupling flow injection electrochemiluminescence(FI-ECL) and a Ru(bpy)32+ECL sensor (Ru(bpy)32+-Nafion-CPE), which was designed bylayer by layer assembling techniques. It is based on the oxidized Ru(bpy)32+on electrodesurface reacting with etamsylate and producing an excellent ECL signal, the ECL intensityof the sensor increased with the concentration of the etamsylate, and the limit of detection(LOD) for etamsylate is0.57ng/mL. The method shows several advantages, such ascost-effectiveness, without reagent consumption, enhancement of ECL and simpleexperimental, which can expand considerably the range of Ru(bpy)32+ECL.In chapter3, in the anode Ru(bpy)32+ECL system, a capillary electrophoresis (CE)coupling to Ru(bpy)32+-based ECL method for the simultaneous determination of atropine,etamsylate, tramadol and lidocaine in a small volume of urine by using β-CD as an additivefor better separation effect was established. There was a linear relationship within a certain range between ECL intensity and the concentration of the four drugs. Thus, a rapid,sensitive and highly selective method has been established for determination ofpostoperative medication. The ECL mechanism has also been discussed in detail.